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公开(公告)号：US20070212709A1
公开(公告)日：2007-09-13
申请号：US11618663
申请日：2006-12-29
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07D311/82 , C07D311/90 , C07D405/12 , C07D405/14 , C07H21/00 , C09B11/04 , C09B11/24 , C09B23/083 , C09B69/00 , C12Q1/6818 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101 , C12Q2565/1015
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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公开(公告)号：US20070207477A1
公开(公告)日：2007-09-06
申请号：US11618688
申请日：2006-12-29
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07H21/00 , C09B11/24 , C12Q1/6818
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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公开(公告)号：US20050112781A1
公开(公告)日：2005-05-26
申请号：US10788660
申请日：2004-02-26
申请人： Linda Lee , Scott Benson , Barnett Rosenblum , Sandra Spurgeon , Ronald Graham
发明人： Linda Lee , Scott Benson , Barnett Rosenblum , Sandra Spurgeon , Ronald Graham
IPC分类号： C07D311/88 , C07D401/06 , C07D401/14 , C07D405/14 , C07D491/14 , C07D491/147 , C12Q1/68 , G01N33/543
CPC分类号： C07D401/06 , C07D401/14 , C07D405/14 , C07D491/147 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101
摘要： A set of 4,7-dichlororhodamine compounds useful as fluorescent dyes are disclosed having the structures wherein R1-R6 are hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group, or, when taken together, R1 and R6 is benzo, or, when taken together, R4 and R5 is benzo; R7-R10, R12-R16 and R18 may be hydrogen, fluorine, chlorine, lower alkyl, lower alkene, lower alkyne, sulfonate, sulfone, amino, amido, nitrile, lower alkoxy, linking group; R11 and R17 may be hydrogen, lower alkyl, lower alkene, lower alkyne, phenyl, aryl, linking group; Y1-Y4 are hydrogen, lower alkyl, or cycloalkyl, or, when taken together, Y1 and R2, Y2 and R1 Y3 and R3, and/or Y4 and R4 is propano, ethano, or substituted forms thereof, and X1-X3 taken separately are hydrogen, chlorine, fluorine, lower alkyl, carboxylate, sulfonate, hydroxymethyl, and linking group, or any combinations thereof. In another aspect, the invention includes reagents labeled with the 4,7-dichlororhodamine dye compounds, including deoxynucleotides, dideoxynucleotides, and polynucleotides. In an additional aspect, the invention includes methods utilizing such dye compounds and reagents including dideoxy polynucleotide sequencing and fragment analysis methods.
摘要翻译： 公开了一组用作荧光染料的4,7-二氯罗丹明化合物，其中R 1 -R 6是氢，氟，氯，低级烷基，低级烯烃 ，低级炔烃，磺酸盐，砜，氨基，酰氨基，腈，低级烷氧基，连接基团，或者当R 1和R 6一起为苯并或 当R 4和R 5一起时，是苯并; R 7 -R 10，R 12 -R 16和R 18可以是 氢，氟，氯，低级烷基，低级烯，低级炔，磺酸，砜，氨基，酰氨基，腈，低级烷氧基，连接基团; R 11和R 17可以是氢，低级烷基，低级烯，低级炔，苯基，芳基，连接基团; Y 1 -Y 4是氢，低级烷基或环烷基，或者当一起为Y 1和R 2时， Y 1，Y 2和R 3，Y 3和R 3，和/或Y 3， R 4和R 4是丙酰基，乙酸或其取代形式，分别取代的X 1 -X 3 - 3是氢， 氯，氟，低级烷基，羧酸酯，磺酸酯，羟甲基和连接基团，或其任何组合。 另一方面，本发明包括用4,7-二氯罗丹明染料化合物标记的试剂，包括脱氧核苷酸，双脱氧核苷酸和多核苷酸。 在另一方面，本发明包括利用这种染料化合物的方法和包括双脱氧多核苷酸测序和片段分析方法的试剂。
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公开(公告)号：US07825237B2
公开(公告)日：2010-11-02
申请号：US12554845
申请日：2009-09-04
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07D311/90
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些接头促进能量转移染料中供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R21Z1C（O）R22R28，其中R21是与供体染料连接的C 1-5烷基，C（O）是羰基 Z1是NH，硫或氧，R22是包含烯烃，二烯烃，炔烃，具有至少一个不饱和键的五元和六元环或连接到羰基碳上的稠环结构的取代基，R28 包括将接头连接到受体染料的官能团。
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公开(公告)号：US20070161027A1
公开(公告)日：2007-07-12
申请号：US11617665
申请日：2006-12-28
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07H21/00 , C09B11/24 , C12Q1/6818
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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公开(公告)号：US20070154927A1
公开(公告)日：2007-07-05
申请号：US11618693
申请日：2006-12-29
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07D311/82 , C07D311/90 , C07D405/12 , C07D405/14 , C07H21/00 , C09B11/04 , C09B11/24 , C09B23/083 , C09B69/00 , C12Q1/6818 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101 , C12Q2565/1015
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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公开(公告)号：US20070154926A1
公开(公告)日：2007-07-05
申请号：US11618683
申请日：2006-12-29
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07H21/00 , C09B11/24 , C12Q1/6818
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些接头促进能量转移染料中供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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公开(公告)号：US20070154925A1
公开(公告)日：2007-07-05
申请号：US11618679
申请日：2006-12-29
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07H21/00 , C09B11/24 , C12Q1/6818
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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公开(公告)号：US20070161026A1
公开(公告)日：2007-07-12
申请号：US11617660
申请日：2006-12-28
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
IPC分类号： C12Q1/68 , G01N33/53 , C07K14/47 , C07D405/02 , C07H21/04
CPC分类号： C07H21/04 , C07D311/82 , C07D311/90 , C07D405/12 , C07D405/14 , C07H21/00 , C09B11/04 , C09B11/24 , C09B23/083 , C09B69/00 , C12Q1/6818 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101 , C12Q2565/1015
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers faciliate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些连接器有助于在能量转移染料中的供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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公开(公告)号：US20070154924A1
公开(公告)日：2007-07-05
申请号：US11617667
申请日：2006-12-28
申请人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
发明人： Linda Lee , Sandra Spurgeon , Barnett Rosenblum
CPC分类号： C07H21/04 , C07D311/82 , C07D311/90 , C07D405/12 , C07D405/14 , C07H21/00 , C09B11/04 , C09B11/24 , C09B23/083 , C09B69/00 , C12Q1/6818 , C12Q1/6869 , C12Q2563/107 , C12Q2535/101 , C12Q2565/1015
摘要： Novel linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye are provided. These linkers facilitate the efficient transfer of energy between a donor and acceptor dye in an energy transfer dye. One of these linkers for linking a donor dye to an acceptor dye in an energy transfer fluorescent dye has the general structure R21Z1C(O)R22R28 where R21 is a C1-5 alkyl attached to the donor dye, C(O) is a carbonyl group, Z1 is either NH, sulfur or oxygen, R22 is a substituent which includes an alkene, diene, alkyne, a five and six membered ring having at least one unsaturated bond or a fused ring structure which is attached to the carbonyl carbon, and R28 includes a functional group which attaches the linker to the acceptor dye.
摘要翻译： 提供了用于将供体染料与能量转移荧光染料中的受体染料连接的新型接头。 这些接头促进能量转移染料中供体和受体染料之间的能量的有效转移。 用于将供体染料与能量转移荧光染料中的受体染料连接的这些接头之一具有通式结构R 21 R 1 C（O）R 22 其中R 21是与供体染料连接的C 1-5烷基，C（O）是羰基（C 1 -C 4）烷基， 基团Z 1是NH，硫或氧，R 22是包括烯烃，二烯烃，炔烃，具有至少一个碳原子的五元和六元环的取代基 不饱和键或与羰基碳连接的稠合环结构，R 28包括将接头连接于受体染料的官能团。
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