公开(公告)号：US20110206715A1

公开(公告)日：2011-08-25

申请号：US12753330

申请日：2010-04-02

申请人： MARCEL BEHR ,  PETER SMALL ,  GARY SCHOOLNIK ,  MICHAEL A. WILSON

发明人： MARCEL BEHR ,  PETER SMALL ,  GARY SCHOOLNIK ,  MICHAEL A. WILSON

IPC分类号： A61K39/04 ,  C12N1/21 ,  A61P37/04 ,  A61P31/06

CPC分类号： C12Q1/689 ,  C12Q2600/156

摘要： Specific genetic deletion are identified in mycobacteria isolates, including variations in the M. tuberculosis genome sequence between isolates, and numerous deletion present in BCG as compared to M. tb. These deletions are used as markers to distinguish between pathogenic and avirulent strains, and as a marker for particular M. tb. isolates. Deletions specific to vaccine strains of BCG are useful in determining whether a positive tuberculin skin test is indicative of actual tuberculosis infection. The deleted sequences may be reintroduced into BCG to improve the efficacy of vaccination. Alternatively, the genetic sequence that corresponds to the deletion(s) are deleted from M. bovis or M. tuberculosis to attenuate the pathogenic bacteria.

摘要翻译： 在分枝杆菌分离株中鉴定了特异性遗传缺失，包括分离株之间的结核分枝杆菌基因组序列的变异，以及与M.bb相比在BCG中存在的许多缺失。 这些缺失被用作区分病原菌和无毒菌株的标记物，并用作特异性M.tb的标记物。 分离株 对BCG疫苗株特异性的缺失可用于确定阳性结核菌素皮肤试验是否表明实际的结核病感染。 可将经删除的序列重新引入BCG以提高疫苗接种的效力。 或者，对应于缺失的遗传序列从牛分枝杆菌或结核分枝杆菌中缺失，以减毒病原菌。