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公开(公告)号:US10125355B2
公开(公告)日:2018-11-13
申请号:US14576793
申请日:2014-12-19
发明人: Fumikazu Yokoyama , Kengo Yokoyama , Nobuko Mazuka
IPC分类号: C12N9/42 , C12P19/14 , D21C5/00 , D21H17/00 , D06M15/15 , A23K10/32 , A23K10/14 , D21H17/22 , D21H21/10
摘要: By combination of hydrophobic chromatography and strongly basic anion-exchange chromatography, a novel, highly hydrophobic β-glucosidase was successfully identified from Acremonium cellulolyticus. Further, a gene corresponding to the identified β-glucosidase was isolated. When multiple modifications were introduced into the base sequence of the gene, the gene was successfully expressed in Trichoderma viride at a high level, and the expression product successfully exhibited a high β-glucosidase activity.