公开(公告)号：US20140366220A1

公开(公告)日：2014-12-11

申请号：US14364184

申请日：2012-12-07

Applicant: Mark D. Kinkema ,  Stacy Miles

Inventor： Mark D. Kinkema ,  Stacy Miles

IPC: C12N15/82

CPC classification number: C12N15/8245 ,  C12N15/8216

Abstract: A sugar cane plant cell comprising a recombinant nucleic acid molecule is provided, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotide sequence of interest, and the nucleotide sequence of interest is expressed at a level at least about 6 times greater than the level of expression of said nucleotide sequence of interest in a control. Additionally, a method of increasing the expression of a nucleotide sequence of interest in a sugar cane plant cell using the recombinant nucleic acid molecule of the invention is provided.

Abstract translation: 提供了包含重组核酸分子的甘蔗植物细胞，所述重组核酸分子包含目的核苷酸序列，启动子和SEQ ID NO：1的核苷酸序列，其中所述启动子位于SEQ ID NO：1的下游且与 SEQ ID NO：1的核苷酸序列并与感兴趣的核苷酸序列上游并且与目标核苷酸序列有效结合，并且感兴趣的核苷酸序列的表达水平比所述核苷酸序列的表达水平至少大约6倍 控制权益。 另外，提供了使用本发明的重组核酸分子增加甘蔗植物细胞中目的核苷酸序列的表达的方法。

公开(公告)号：US09441234B2

公开(公告)日：2016-09-13

申请号：US14364184

申请日：2012-12-07

Applicant: Mark D. Kinkema ,  Stacy Miles

Inventor： Mark D. Kinkema ,  Stacy Miles

IPC: C12N15/82

CPC classification number: C12N15/8245 ,  C12N15/8216

Abstract: A sugar cane plant cell comprising a recombinant nucleic acid molecule is provided, the recombinant nucleic acid molecule comprising a nucleotide sequence of interest, a promoter and the nucleotide sequence of SEQ ID NO:1, wherein the promoter is downstream of and in operative association with the nucleotide sequence of SEQ ID NO:1 and upstream of and in operative association with the nucleotide sequence of interest, and the nucleotide sequence of interest is expressed at a level at least about 6 times greater than the level of expression of said nucleotide sequence of interest in a control. Additionally, a method of increasing the expression of a nucleotide sequence of interest in a sugar cane plant cell using the recombinant nucleic acid molecule of the invention is provided.

Abstract translation: 提供了包含重组核酸分子的甘蔗植物细胞，所述重组核酸分子包含目的核苷酸序列，启动子和SEQ ID NO：1的核苷酸序列，其中所述启动子位于SEQ ID NO：1的下游且与 SEQ ID NO：1的核苷酸序列并与感兴趣的核苷酸序列上游并且与目标核苷酸序列有效结合，并且感兴趣的核苷酸序列的表达水平比所述核苷酸序列的表达水平至少大约6倍 控制权益。 另外，提供了使用本发明的重组核酸分子增加甘蔗植物细胞中目的核苷酸序列的表达的方法。