公开(公告)号：US20240044833A1

公开(公告)日：2024-02-08

申请号：US18026043

申请日：2021-09-14

Applicant: McMaster University

Inventor： Leyla Soleymani ,  Sarah Traynor ,  Feng Li

IPC: G01N27/327 ,  G01N33/53 ,  G01N33/543

CPC classification number: G01N27/3276 ,  G01N33/5308 ,  G01N33/5438 ,  G01N27/3277

Abstract: This disclosure relates to a biosensor for detecting a target analyte in a sample comprising: a double-stranded oligonucleotide comprising an overhang on a first strand of the oligonucleotide, and a second strand of the oligonucleotide that is a reporter moiety comprising a detectable label; a first detection probe comprising a recognition moiety and a junction forming moiety, wherein the junction forming moiety comprises a first portion capable of binding by complementarity to the overhang of the first strand of the double-stranded oligonucleotide; a second detection probe comprising a recognition moiety and a junction forming moiety, wherein the junction forming moiety comprises a first portion capable of binding by complementarity to an internal segment of the first strand of the double-stranded oligonucleotide; and a capture probe functionalized on an electrode, wherein the capture probe comprises an immobilized strand attached to the electrode. Methods and uses thereof are also disclosed herein.