公开(公告)号：US08383879B2

公开(公告)日：2013-02-26

申请号：US12959632

申请日：2010-12-03

申请人： Mi-Hua Tao ,  Cheng-Chieh Fang ,  Ya-Hui Huang

发明人： Mi-Hua Tao ,  Cheng-Chieh Fang ,  Ya-Hui Huang

IPC分类号： A01K67/00 ,  C12N15/864

CPC分类号： G01N33/5088 ,  G01N33/5011 ,  G01N2333/02

摘要： A non-human animal disease model for hepatitis B virus-associated liver disease is disclosed. The animal disease model is transduced with a hepatitis B virus genome in the liver cells thereof and exhibits the following symptoms: hepatitis B viral particles and hepatitis B viral DNA in the serum, hepatitis B virus (HBV) envelope and HBV e proteins in the serum, expression of HBV core and HBV envelope proteins in the liver but not in the kidney, heart, lung, brain, pancreas, spleen, stomach or intestine tissues. The animal disease model may develop hepatocellular carcinoma, exhibiting an elevated level of alanine aminotransferase as compared to a control animal without the hepatitis B virus genome in the liver cells thereof, and liver pathological symptoms such as tumor nodules, dysplasia, inflammatory infiltrates, necrosis and fibrosis.

摘要翻译： 公开了一种用于乙型肝炎病毒相关肝病的非人动物疾病模型。 动物疾病模型在其肝细胞中用乙型肝炎病毒基因组转导并表现出以下症状：血清中的乙型肝炎病毒颗粒和乙型肝炎病毒DNA，乙型肝炎病毒（HBV）包膜和血清中的HBV e蛋白 ，HBV核心和HBV包膜蛋白在肝脏中表达，但不在肾脏，心脏，肺，脑，胰腺，脾脏，胃或肠组织中表达。 动物疾病模型可能发展为肝细胞癌，与肝细胞中没有乙型肝炎病毒基因组的对照动物相比，表现出高水平的丙氨酸氨基转移酶，以及肝脏病理学症状如肿瘤结节，发育不良，炎性浸润，坏死和 纤维化。