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    Method, Particularly Enzyme-Linked Immunosorbent Assay (ELISA), for In Vitro Detection of Amyloid Beta Autoantibodies, Microtiter Plate, and Test Kit
    1.
    发明申请
    Method, Particularly Enzyme-Linked Immunosorbent Assay (ELISA), for In Vitro Detection of Amyloid Beta Autoantibodies, Microtiter Plate, and Test Kit 审中-公开
    方法，特别是酶联免疫吸附测定（ELISA），用于体外检测淀粉样蛋白β自身抗体，微量滴定板和测试试剂盒

    公开(公告)号：US20130109033A1

    公开(公告)日：2013-05-02

    申请号：US13384726

    申请日：2010-07-14

    申请人： Michael Bacher ,  Richard Dodel ,  Karthikeyan Balakrishnan

    发明人： Michael Bacher ,  Richard Dodel ,  Karthikeyan Balakrishnan

    IPC分类号： G01N33/53

    CPC分类号： G01N33/5306 ,  B01L3/5085 ,  B01L2300/0829 ,  G01N33/54386 ,  G01N33/564 ,  G01N33/6896 ,  G01N2800/2821

    摘要： A method, particularly an enzyme-linked immunosorbent assay (ELISA), for the in-vitro detection of Aβ autoantibodies in human serum and/or plasma contains the following steps: preparing an antigen-coated solid phase; incubating the solid phase with a blocking solution; incubating the solid phase with a sample to be examined; immunological detection of the Aβ autoantibodies on the solid phase; and reading the detected results of the solid phase using a reading tool. According to the invention, the preparation of the antigen-coated solid phase advantageously includes incubating the solid phase with a coating solution in which the antigen is dissolved, said antigen having a peptide sequence selected from the group SEQ ID No. 1, SEQ ID No. 2 or SEQ ID No. 3.

    摘要翻译： 用于体外检测人血清和/或血浆中Abeta自身抗体的方法，特别是酶联免疫吸附测定法（ELISA）包括以下步骤：制备抗原包被的固相; 用固定溶液孵育固相; 将固相与待检样品孵育; Abeta自身抗体在固相上的免疫学检测; 并使用读取工具读取检测到的固相的结果。 根据本发明，抗原包被的固相的制备有利地包括将固相与其中溶解抗原的包被溶液一起孵育，所述抗原具有选自SEQ ID No.1，SEQ ID No 2或SEQ ID No.3。