公开(公告)号：US07846896B2

公开(公告)日：2010-12-07

申请号：US11869797

申请日：2007-10-10

申请人： Michael Brandt ,  Apollon Papdimitriou

发明人： Michael Brandt ,  Apollon Papdimitriou

IPC分类号： A61K38/18 ,  C07K38/00

CPC分类号： A61K47/60

摘要： The present invention provides a conjugate consisting essentially of a NK4 molecule and a polyethylene glycol group having a molecular weight of from about 20 to about 40 kDa. The invention also provides a composition in which the monoPEGylated conjugates comprise at least 90% of the total of pegylated NK4 molecules and unpegylated NK4 molecules in the composition. Also provided is a composition in which the monoPEGylated conjugates comprise conjugates in which the PEG groups are attached to groups randomly selected from the lysine side chains of NK4 molecules and the N-terminal amino groups of NK4 molecules. A method for the treatment of cancer by administering 1 to 30 mg monoPEGylated NK4 per kg per day is further provided.

摘要翻译： 本发明提供了基本上由NK4分子和分子量为约20至约40kDa的聚乙二醇基团组成的缀合物。 本发明还提供了一种组合物，其中单聚乙二醇化缀合物包含组合物中聚乙二醇化NK4分子和未聚乙二醇化NK4分子总数的至少90％。 还提供了其中单PEG化缀合物包含缀合物的组合物，其中PEG基团连接到随机选自NK4分子的赖氨酸侧链和NK4分子的N-末端氨基的基团。 进一步提供通过每公斤施用1至30mg单PEG化的NK4来治疗癌症的方法。