公开(公告)号：US06509161B1

公开(公告)日：2003-01-21

申请号：US09514994

申请日：2000-02-29

申请人： Michael Gordon Barker ,  Nicholas Billinton ,  Nicholas John Goddard ,  Richard Maurice Walmsley ,  Andrew William Knight ,  Peter Robert Fielden

发明人： Michael Gordon Barker ,  Nicholas Billinton ,  Nicholas John Goddard ,  Richard Maurice Walmsley ,  Andrew William Knight ,  Peter Robert Fielden

IPC分类号： G01N3353

CPC分类号： G01N33/582

摘要： A method of distinguishing between fluorescent light emitted by green fluorescent protein and fluorescent light emitted by auto-fluorescent molecules, the method comprising: using plane-polarised light to illuminate a sample containing green fluorescent protein and auto-fluorescent molecules; detecting the intensity of fluorescent light that is emitted with a first polarisation from the sample; detecting the intensity of fluorescent light that is emitted with a second polarisation from the sample; and subtracting a first of said detected intensities from a second of said detected intensities to obtain a difference signal.

摘要翻译： 一种鉴别绿色荧光蛋白发出的荧光和由自身荧光分子发出的荧光的方法，其特征在于，使用平面偏振光照射含有绿色荧光蛋白和自身荧光分子的样品，检测荧光强度 用来自样品的第一极化发射的光;检测来自样品的以第二极化发射的荧光的强度; 并且从所述检测到的强度的第二个中减去所述检测到的强度中的第一个，以获得差分信号。