Immunoassay technique for specific immunoglobulin E
    1.
    发明授权
    Immunoassay technique for specific immunoglobulin E 失效
    特异性免疫球蛋白E的免疫测定技术

    公开(公告)号:US4539292A

    公开(公告)日:1985-09-03

    申请号:US454219

    申请日:1982-12-29

    IPC分类号: G01N33/68 G01N33/54

    摘要: Using monoclonal mouse anti-human IgE antibodies, the microtiter solid phase radioimmunoassay (MSPRIA) and the enzyme-linked immunoabsorbent assay (EILSA) were modified to quantitatively measure honeybee venom (HBV) and perennial rye grass (PRG) specific immunoglobulin E (sIgE). By using a novel dilution, serial transfer and cummulative counting technique, the inhibiting and interfering effects of specific immunoglobulin G (sIgG) in the assay of (sIgE) can be reduced and the quantitative determination of (sIgE) accomplished with precision and reliability.

    摘要翻译: 使用单克隆小鼠抗人IgE抗体,修饰微量滴定固相放射免疫测定(MSPRIA)和酶联免疫吸附测定(EILSA),定量测定蜜蜂毒液(HBV)和多年生黑麦草(PRG)特异性免疫球蛋白E(sIgE) 。 通过使用新型稀释,连续转移和累积计数技术,可以减少特异性免疫球蛋白G(sIgG)在(sIgE)测定中的抑制和干扰作用,并以精确和可靠的方式实现(sIgE)的定量测​​定。