公开(公告)号：US07060465B2

公开(公告)日：2006-06-13

申请号：US11235009

申请日：2005-09-26

申请人： Ming-qun Xu ,  Sebastien M. Ferrandon ,  Christopher H. Taron ,  Paul A. Colussi

发明人： Ming-qun Xu ,  Sebastien M. Ferrandon ,  Christopher H. Taron ,  Paul A. Colussi

IPC分类号： C12P21/00

CPC分类号： C12N15/80 ,  C12N9/2442 ,  C12Y302/01014

摘要： Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

公开(公告)号：US06897285B2

公开(公告)日：2005-05-24

申请号：US10375913

申请日：2003-02-26

申请人： Ming-Qun Xu ,  Sebastien M. Ferrandon ,  Christopher H. Taron ,  Paul A. Colussi

发明人： Ming-Qun Xu ,  Sebastien M. Ferrandon ,  Christopher H. Taron ,  Paul A. Colussi

IPC分类号： C12N9/24 ,  C07K14/00

CPC分类号： C12N15/80 ,  C12N9/2442 ,  C12Y302/01014

摘要： Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

摘要翻译： 提供了组合物和方法，用于在非变性条件下将壳多糖结合域（CBD）可逆地结合到壳多糖或等效底物。 CBD优选通过突变来改变以实现这种性质的变化。 在一个实施方案中，位于CBD的结合裂缝中的芳族氨基酸残基被改变，导致在选择的条件下对底物的可逆结合亲和力。 用改良的底物结合亲和力建立经修饰的CBD，为以前不可能与未结合的CBD结合的CBD提供了新的用途，后者不可逆地与几丁质结合。

公开(公告)号：US06987007B2

公开(公告)日：2006-01-17

申请号：US11110002

申请日：2005-04-20

申请人： Ming-Qun Xu ,  Sebastien M. Ferrandon

发明人： Ming-Qun Xu ,  Sebastien M. Ferrandon

IPC分类号： C12P21/00

CPC分类号： C12N15/80 ,  C12N9/2442 ,  C12Y302/01014

摘要： Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

公开(公告)号：US06984505B2

公开(公告)日：2006-01-10

申请号：US11110001

申请日：2005-04-20

申请人： Ming-Qun Xu ,  Sebastien M. Ferrandon

发明人： Ming-Qun Xu ,  Sebastien M. Ferrandon

IPC分类号： C12P21/00

CPC分类号： C12N15/80 ,  C12N9/2442 ,  C12Y302/01014

摘要： Compositions and methods are provided for reversibly binding chitin binding domain (CBD) to a chitin or equivalent substrate under non denaturing conditions. CBD is modified preferably by a mutation to achieve this change in properties. In one embodiment, an aromatic amino acid residue located in the binding cleft of the CBD was altered resulting in reversible binding affinity for substrate in select conditions. Creating a modified CBD with an altered binding affinity for substrate provides new uses for CBD not previously possible with unmodified CBD which binds irreversibly to chitin.

摘要翻译： 提供了组合物和方法，用于在非变性条件下将壳多糖结合域（CBD）可逆地结合到壳多糖或等效底物。 CBD优选通过突变来改变以实现这种性质的变化。 在一个实施方案中，位于CBD的结合裂缝中的芳族氨基酸残基被改变，导致在选择的条件下对底物的可逆结合亲和力。 用改良的底物结合亲和力建立经修饰的CBD，为以前不可能与未结合的CBD结合的CBD提供了新的用途，后者不可逆地与几丁质结合。