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公开(公告)号：US20090075310A1
公开(公告)日：2009-03-19
申请号：US12265202
申请日：2008-11-05
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholesterols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： 一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以便优先反应高密度和非常低密度的胆固醇中的胆固醇 在脂蛋白中，然后确定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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公开(公告)号：US20130078659A1
公开(公告)日：2013-03-28
申请号：US13679594
申请日：2012-11-16
IPC分类号： C12Q1/60
CPC分类号： C12Q1/60
摘要： A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers.
摘要翻译： 一种定量测定LDL胆固醇的方法，包括以下步骤：向血清中加入选自聚氧乙烯亚烷基苯基醚和聚氧乙烯亚烷基三苄基苯基醚的表面活性剂和胆固醇分析酶试剂，以优先反应高密度和非常低密度的胆固醇中的胆石甾醇 在脂蛋白中，然后确定其后反应的胆固醇的量。 该方法可以消除离心和电泳等预处理的必要性，能够以高效，简单的方式进行定量测定，可以应用于各种自动分析装置。
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