公开(公告)号：US07709666B2

公开(公告)日：2010-05-04

申请号：US10647517

申请日：2004-01-16

申请人： Mylavarapu Venkatramesh ,  David R. Corbin ,  B. Ganesh Bhat ,  Sekhar S. Boddupalli ,  Robert J. Grebenok ,  Ganesh M. Kishore ,  Kathryn D. Lardizabal ,  Michael W. Lassner ,  Shaukat H. Rangwala ,  Balasulojini Karunanandaa

发明人： Mylavarapu Venkatramesh ,  David R. Corbin ,  B. Ganesh Bhat ,  Sekhar S. Boddupalli ,  Robert J. Grebenok ,  Ganesh M. Kishore ,  Kathryn D. Lardizabal ,  Michael W. Lassner ,  Shaukat H. Rangwala ,  Balasulojini Karunanandaa

IPC分类号： C07C59/147

CPC分类号： C12N9/001 ,  C12N15/8243

摘要： Provided are recombinant constructs comprising DNA sequences encoding enzymes effective in altering the biosynthesis and accumulation of sterol compounds and tocopherols in transgenic plants. Also provided are methods of using such constructs to produce transgenic plants, seeds of which contain elevated levels of sitostanol and/or sitostanol esters, and α-tocopherol, as well as reduced levels of campesterol and campestanol and their corresponding esters. These seeds also contain the novel sterol brassicastanol. Oil obtained from seeds of such transgenic plants is also provided. This oil can be used to prepare food and pharmaceutical compositions effective in lowering the level of low density lipoprotein cholesterol in blood serum. In addition, novel DNA sequences encoding plant steroid 5α-reductases are also disclosed.

摘要翻译： 提供了包含编码有效改变转基因植物中甾醇化合物和生育酚的生物合成和积累的酶的DNA序列的重组构建体。 还提供了使用这种构建体产生转基因植物的方法，其种子含有升高水平的谷甾烷醇和/或谷甾烷醇酯和α-生育酚，以及降低水平的菜油甾醇和菜油甾醇及其相应的酯。 这些种子还含有新的甾醇黄豆糖醇。 还提供了从这种转基因植物的种子获得的油。 该油可用于制备有效降低血清中低密度脂蛋白胆固醇水平的食品和药物组合物。 此外，还公开了编码植物类固醇5α-还原酶的新型DNA序列。

公开(公告)号：US20100100987A1

公开(公告)日：2010-04-22

申请号：US12634356

申请日：2009-12-09

申请人： Barkur G. BHAT ,  Sekhar S. Boddupalli ,  Ganesh M. Kishore ,  Jingdong Liu ,  Shaukat H. Rangwala ,  Mylavarapu Venkatramesh

发明人： Barkur G. BHAT ,  Sekhar S. Boddupalli ,  Ganesh M. Kishore ,  Jingdong Liu ,  Shaukat H. Rangwala ,  Mylavarapu Venkatramesh

IPC分类号： A01H5/00 ,  C07H21/04 ,  A01C7/00

CPC分类号： C12N15/8243 ,  C12N9/0069 ,  C12N15/8247 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize and soybean associated with the tocopherol synthesis pathway enzymes. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression and transgenic plants.

摘要翻译： 本发明在植物生物化学领域。 更具体地，本发明涉及来自植物细胞的核酸序列，特别是与生育酚合成途径酶相关的玉米和大豆的核酸序列。 本发明包括编码蛋白质和蛋白质片段的核酸分子。 此外，本发明还包括如此编码的蛋白质和蛋白质的片段以及能够结合这些蛋白质或片段的抗体。 本发明还涉及使用核酸分子，蛋白质和蛋白质和抗体片段的方法，例如用于基因组图谱，基因鉴定和分析，植物育种，用于植物基因表达的构建体的制备和转基因植物。

公开(公告)号：US07655777B2

公开(公告)日：2010-02-02

申请号：US11329160

申请日：2006-01-11

申请人： Barkur G. Bhat ,  Sekhar S. Boddupalli ,  Ganesh M. Kishore ,  Jingdong Liu ,  Shaukat H. Rangwala ,  Mylavarapu Venkatramesh

发明人： Barkur G. Bhat ,  Sekhar S. Boddupalli ,  Ganesh M. Kishore ,  Jingdong Liu ,  Shaukat H. Rangwala ,  Mylavarapu Venkatramesh

IPC分类号： C07H21/02 ,  C12Q1/66

CPC分类号： C12N15/8243 ,  C12N9/0069 ,  C12N15/8247 ,  C12Q1/6895 ,  C12Q2600/156

摘要： The present invention is in the field of plant biochemistry. More specifically the invention relates to nucleic acid sequences from plant cells, in particular, nucleic acid sequences from maize and soybean associated with the tocopherol synthesis pathway enzymes. The invention encompasses nucleic acid molecules that encode proteins and fragments of proteins. In addition, the invention also encompasses proteins and fragments of proteins so encoded and antibodies capable of binding these proteins or fragments. The invention also relates to methods of using the nucleic acid molecules, proteins and fragments of proteins and antibodies, for example for genome mapping, gene identification and analysis, plant breeding, preparation of constructs for use in plant gene expression and transgenic plants.

摘要翻译： 本发明在植物生物化学领域。 更具体地，本发明涉及来自植物细胞的核酸序列，特别是与生育酚合成途径酶相关的玉米和大豆的核酸序列。 本发明包括编码蛋白质和蛋白质片段的核酸分子。 此外，本发明还包括如此编码的蛋白质和蛋白质的片段以及能够结合这些蛋白质或片段的抗体。 本发明还涉及使用核酸分子，蛋白质和蛋白质和抗体片段的方法，例如用于基因组图谱，基因鉴定和分析，植物育种，用于植物基因表达的构建体的制备和转基因植物。

公开(公告)号：US06653530B1

公开(公告)日：2003-11-25

申请号：US09023587

申请日：1998-02-13

申请人： Christine K. Shewmaker ,  B. Ganesh Bhat ,  Mylavaraapu Venkatramesh ,  Shaukat H. Rangwala ,  Ganesh M. Kishore ,  Sekhar S. Boddupalli

发明人： Christine K. Shewmaker ,  B. Ganesh Bhat ,  Mylavaraapu Venkatramesh ,  Shaukat H. Rangwala ,  Ganesh M. Kishore ,  Sekhar S. Boddupalli

IPC分类号： A01H500

CPC分类号： C12N15/825 ,  C12N15/8243 ,  C12N15/8247

摘要： Methods are provided for producing plants and seeds having altered carotenoid, fatty acid and tocopherol compositions. The methods find particular use in increasing the carotenoid and tocopherol levels in oilseed plants, and in providing desirable high oleic acid seed oils.

摘要翻译： 提供了用于生产具有改变的类胡萝卜素，脂肪酸和生育酚组合物的植物和种子的方法。 该方法特别用于增加油籽植物中的类胡萝卜素和生育酚水平，以及提供所需的高油酸种子油。