公开(公告)号：US20220204923A1

公开(公告)日：2022-06-30

申请号：US17582730

申请日：2022-01-24

Applicant: NANJING JOES FUTURE FOOD TECHNOLOGY CO., LTD.

Inventor： Guanghong ZHOU ,  Zhongyuan WU ,  Xinglian XU ,  Shijie DING ,  Huixia LI

IPC: C12N5/0735

Abstract: A chemically defined medium for differentiation of muscle stem cells in vitro, namely a serum-free, more efficient and inexpensive chemically defined medium for inducing differentiation of muscle stem cells in vitro. Compared with an existing general muscle stem cell differentiation medium, using the chemically defined medium can increase the relative expression of myogenin genes by 4.48 times on the 2nd day of differentiation, increase the relative expression of myosin heavy chain genes by 55.28 times on the 6th day, and increase the percentage of cell differentiation from 34.94% to 57.93% in the terminal differentiation stage, and more, thicker and longer muscle fibers are formed through induced differentiation. The chemically defined differentiation medium further improves the differentiation efficiency of muscle stem cells, and provides a more efficient and inexpensive method for differentiation of muscle stem cells into myotubes, and for 3D culture of muscle stem cells to produce cell cultured meat.

公开(公告)号：US20250151771A1

公开(公告)日：2025-05-15

申请号：US18838612

申请日：2023-06-30

Applicant: NANJING JOES FUTURE FOOD TECHNOLOGY CO., LTD.

Inventor： Guanghong ZHOU ,  Jie WANG ,  Xi DING ,  Shijie DING

IPC: A23P20/20 ,  A23L13/00 ,  A23P20/25 ,  B33Y10/00 ,  B33Y30/00 ,  B33Y50/02 ,  B33Y80/00 ,  C12M1/00 ,  C12M1/26 ,  C12M3/00 ,  C12M3/06 ,  C12N5/077

Abstract: A cell-cultured meat production device based on microfluidic 3D printing technology includes a printing nozzle, a printing moving system, a loading platform, a sample injection system and a base. The printing moving system is disposed on the base and is composed of a plurality of movable optical axes. The printing nozzle is disposed on a movable optical axis, the sample injection system is connected to the printing nozzle, and the printing nozzle is a micro-fluidic chip. The present invention is based on microfluidic 3D printing, where biological ink is extruded by means of a microfluidic chip to form printed fibers that, under the drive of the printing moving system, are stacked for formation to achieve single-step construction of a piece of three-dimensional tissue having tissue anisotropy, which is used for cell-cultured meat production.