公开(公告)号：US20170088884A1

公开(公告)日：2017-03-30

申请号：US15126178

申请日：2015-01-29

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Chun WU

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/44 ,  C12Q1/6869 ,  C12Y301/21 ,  C12Y301/21005 ,  C12Q2525/119

Abstract: The present invention provides a method for detecting the presence or absence of a guanine-abasic site, the method being a process for detecting guanine opposite at least one abasic sites generated in a double-stranded DNA, comprising: (1) step 1 of site-selectively cleaving at least one abasic sites in a double-stranded DNA using an enzyme; (2) step 2 of modifying the amino group at position 2 of guanine opposite the abasic sites using a modifier; and (3) step 3 of performing polymerase chain reaction on the modified double-stranded DNA obtained by conducting step 1 and step 2, which serves as a template, to search for the presence or absence of an amplification product, the sequence of steps 1 and 2 being not limited to the order presented.