公开(公告)号：US20040038367A1

公开(公告)日：2004-02-26

申请号：US10395241

申请日：2003-03-25

Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY

Inventor： Katsuro Yaoi ,  Yasushi Mitsuishi

IPC: C12P019/04 ,  C07H021/04 ,  C08B037/00 ,  C12N009/24 ,  C12P021/02 ,  C12N005/06

CPC classification number: C12N9/2434

Abstract: The present invention provides a novel xyloglucan oligosaccharide-degrading enzyme having a different degradation mechanism from known enzymes. This enzyme specifically cleaves the second null-glucoside linkage, counted from the reducing end among the null-glucoside linkage constituting the principal chain of xyloglucan oligosaccharide. In the present invention, the enzyme is collected from microorganisms belonging to Geotrichum species, and the amino acid and base sequences of the enzyme and polynucleotide encoding the enzyme are determined. Thus, high-purity polypeptides having the xyloglucan oligosaccharide-degradation activity can be prepared at low cost through a genetic engineering process.

Abstract translation: 本发明提供了与已知酶具有不同降解机理的新型木葡聚寡糖降解酶。 该酶特异性切割第二个β-葡萄糖苷键，从构成木葡聚寡糖主链的β-葡萄糖苷键中的还原末端计数。 在本发明中，从属于地热属物种的微生物收集酶，并测定酶和编码该酶的多核苷酸的氨基酸和碱基序列。 因此，可以通过遗传工程方法以低成本制备具有木葡聚寡糖降解活性的高纯度多肽。