公开(公告)号：US20230056355A1

公开(公告)日：2023-02-23

申请号：US17750241

申请日：2022-05-20

Applicant: NEURACLE GENETICS INC.

Inventor： Joo Seok HAN ,  Hoon Young KONG ,  Yoon Hyung HWANG

IPC: C12N15/86

Abstract: The present disclosure relates to a dual helper plasmid for producing a recombinant adeno-associated virus. A double transfection method using the dual helper plasmid of the present disclosure is advantageous over the triple transfection method typically used for production of adeno-associated virus in terms of 1) increased chance of co-transfection, 2) increased productivity of recombinant adeno-associated virus, 3) reduction in cost and time of plasmid production and purification, etc., and thus can be usefully utilized for effective production of a gene therapy agent.