公开(公告)号：US11530416B2

公开(公告)日：2022-12-20

申请号：US17548597

申请日：2021-12-13

Applicant: NINGBO UNIVERSITY

Inventor： Xingfeng Shao ,  Yingying Wei ,  Yihui Mao ,  Yi Chen ,  Shu Jiang

IPC: C12N15/82

Abstract: The present invention discloses a Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene, and a cloning method and use thereof. The Prunus persica polygalacturonase-inhibiting protein 1 PpPGIP1 gene has a nucleotide sequence shown in SEQ ID NO: 1, and a protein encoded by the Prunus persica polygalacturonase-inhibiting protein 1 (PpPGIP1) gene has an amino acid sequence shown in SEQ ID NO: 2. The cloning method includes the following steps: (1) extracting total RNA from a peach, and subjecting the total RNA to reverse transcription to obtain cDNA, which serves as a template; (2) designing primers based on the PpPGIP1 gene sequence; and (3) Polymerase Chain Reaction (PCR) amplification: conducting PCR amplification to obtain a PpPGIP1 gene amplification product.