公开(公告)号：US20220365102A1

公开(公告)日：2022-11-17

申请号：US17764931

申请日：2020-09-02

Applicant: NIPRO CORPORATION

Inventor： Yoshinori KOSOKU ,  Yuka SANO ,  Toshiaki BABA ,  Hiroshi YOSHIDA

IPC: G01N33/68 ,  G01N33/543

Abstract: Two different antibodies that specifically bind to tau protein or phosphorylated tau protein are used. One of the two different antibodies is a first antibody immobilized on a support or labeled with a molecule capable of binding to the support, and the other of the two different antibodies is a second antibody labeled without being immobilized on the support. The tau protein includes an N-terminal domain, a C-terminal domain, and an intermediate domain located between the N-terminal and C-terminal domains. Epitopes recognized by the first and second antibodies are each an amino acid sequence contained in the intermediate domain or an amino acid sequence contained in the N-terminal domain. One of the first and second antibodies is first subjected to an antigen-antibody reaction with the blood sample, and the other of the first and second antibodies is subsequently subjected to an antigen-antibody reaction with the blood sample.