公开(公告)号：US20150203807A1

公开(公告)日：2015-07-23

申请号：US14412931

申请日：2013-07-02

Applicant: NOVOZYMES A/S

Inventor： Helene Munthe Jensen ,  Martin Ellegaard ,  Sune Jakobsen

IPC: C12N1/00 ,  C12N9/42

CPC classification number: C12N1/005 ,  A01N37/02 ,  C12N9/2434 ,  C12N9/2437

Abstract: A method of inactivating the microbial host cell in a fermentation broth comprising an enzyme of interest and the microbial host cell producing the enzyme of interest comprising: a) Adding a fatty acid having a chain length of C4-C12 to the fermentation broth; and b) Mixing the fermentation broth for a sufficient period of time until the microbial host cell is inactivated.

Abstract translation: 在包含感兴趣的酶的发酵液中灭活微生物宿主细胞的方法和产生感兴趣的酶的微生物宿主细胞的方法包括：a）向发酵液中加入链长为C4-C12的脂肪酸; 和b）将发酵液混合足够的时间直到微生物宿主细胞灭活。