公开(公告)号：US20150093415A1

公开(公告)日：2015-04-02

申请号：US14394986

申请日：2013-02-04

Applicant: National Center for Nanoscience and Technology

Inventor： Guangjun Nie ,  Xin Tian ,  Motao Zhu

IPC: A61K39/00 ,  C12N5/0784

CPC classification number: A61K39/0011 ,  A61K2039/5154 ,  A61K2039/5158 ,  C12N5/0639 ,  C12N2501/052 ,  C12N2501/22 ,  C12N2501/2304

Abstract: The present invention provides a preparation method of an antigen composition. The preparation method comprises the following steps: (1) obtaining a tumor antigen protein; (2) making the tumor antigen protein into contact with an immature dendritic cell; (3) inducing the immature dendritic cell in contact with the tumor antigen into a mature dendritic cell; and (4) separating a cell vesicle of the mature dendritic cell. The present invention further provides an antigen composition obtained through the preparation method and the application thereof in preparing a tumor vaccine.

Abstract translation: 本发明提供抗原组合物的制备方法。 制备方法包括以下步骤：（1）获得肿瘤抗原蛋白; （2）使肿瘤抗原蛋白与未成熟树突状细胞接触; （3）将与肿瘤抗原接触的未成熟树突状细胞诱导成成熟树突状细胞; 和（4）分离成熟树突状细胞的细胞囊泡。 本发明还提供通过制备方法获得的抗原组合物及其在制备肿瘤疫苗中的应用。

公开(公告)号：US09526771B2

公开(公告)日：2016-12-27

申请号：US14394986

申请日：2013-02-04

Applicant: National Center for Nanoscience and Technology

Inventor： Guangjun Nie ,  Xin Tian ,  Motao Zhu

IPC: A61K39/00 ,  C12N5/0784

CPC classification number: A61K39/0011 ,  A61K2039/5154 ,  A61K2039/5158 ,  C12N5/0639 ,  C12N2501/052 ,  C12N2501/22 ,  C12N2501/2304

Abstract: The present invention provides a preparation method of an antigen composition. The preparation method comprises the following steps: (1) obtaining a tumor antigen protein; (2) making the tumor antigen protein into contact with an immature dendritic cell; (3) inducing the immature dendritic cell in contact with the tumor antigen into a mature dendritic cell; and (4) separating a cell vesicle of the mature dendritic cell. The present invention further provides an antigen composition obtained through the preparation method and the application thereof in preparing a tumor vaccine.

Abstract translation: 本发明提供抗原组合物的制备方法。 制备方法包括以下步骤：（1）获得肿瘤抗原蛋白; （2）使肿瘤抗原蛋白与未成熟树突状细胞接触; （3）将与肿瘤抗原接触的未成熟树突状细胞诱导成成熟树突状细胞; 和（4）分离成熟树突状细胞的细胞囊泡。 本发明还提供通过制备方法获得的抗原组合物及其在制备肿瘤疫苗中的应用。