公开(公告)号：US11773376B2

公开(公告)日：2023-10-03

申请号：US16630188

申请日：2018-07-12

申请人： National University Corporation Tokyo Medical and Dental University

发明人： Hiroshi Asahara ,  Kensuke Kataoka ,  Tomoki Chiba

IPC分类号： C12N5/077 ,  A61L27/22 ,  A61L27/36 ,  A61L27/38

CPC分类号： C12N5/066 ,  A61L27/225 ,  A61L27/3683 ,  A61L27/386 ,  A61L27/3834 ,  A61L2430/10 ,  C12N2506/45 ,  C12N2527/00 ,  C12N2533/54 ,  C12N2533/56

摘要： The present invention relates to providing artificial tendon or ligament tissue having sufficient strength. More specifically, artificial tendon or ligament tissue having sufficient strength is provided by embedding collagen-secreting cells in a gel having strength capable of resisting a tensile load and by culturing the cells while applying a tensile load to the gel to produce artificial tendon or ligament tissue. Cells that steadily express the Mkx gene can be used as the collagen-secreting cells. A fibrin gel containing aprotinin can be used as the gel.