公开(公告)号：US20110121204A1

公开(公告)日：2011-05-26

申请号：US13055854

申请日：2009-05-20

申请人： Nobutaka Kumazaki ,  Satoshi Takahashi ,  Hirokazu Kato ,  Takanovu Haga

发明人： Nobutaka Kumazaki ,  Satoshi Takahashi ,  Hirokazu Kato ,  Takanovu Haga

IPC分类号： G01J1/58

CPC分类号： G01N21/648 ,  G02B21/16

摘要： A technique and device for fluorescence observation with good operability, high sensitivity, acid high reliability. The device is used for fluorescence observation using evanescent light. The angle of incidence of the excitation light is adjusted so that the excitation light is always totally reflected from the surface of a substrate irrespective of the angle of the surface of the substrate. The method includes a step of shining the excitation light on the observation substrate while continuously varying the angle of the excitation light with respect to the observation substrate, a step of sensing the shone excitation light by means of optical sensors, and a step of setting the angle of total reflection according to the result of the sensing by the optical sensors. The direction in which the shone excitation light travels varies with the angle of incidence. That is, the excitation light travels as the transmitted light, the reflected light, or the surface propagating light. These lights are sensed by the corresponding optical sensors, and how the angle of incidence of the excitation light is with respect to the critical angle is determined. The angle of incidence of the excitation light is varied depending on the result of the determination, thereby realizing an optimum total reflection angle.

摘要翻译： 用于荧光观察的技术和装置，操作性好，灵敏度高，酸度高可靠性。 该器件用于使用ev逝光的荧光观察。 调节激发光的入射角，使得激发光始终从基板的表面全反射，而与基板的表面的角度无关。 该方法包括在观察基板上照射激发光的步骤，同时连续地改变激发光相对于观察基板的角度，通过光学传感器感测照射激发光的步骤，以及将 根据光学传感器的感测结果，全反射角度。 照射激发光的行进方向随入射角而变化。 也就是说，激发光作为透射光，反射光或表面传播光行进。 这些光被相应的光学传感器感测，并且确定激发光的入射角相对于临界角如何。 激发光的入射角取决于确定的结果而变化，从而实现最佳的全反射角。