公开(公告)号：US20230272353A1

公开(公告)日：2023-08-31

申请号：US16971174

申请日：2019-02-21

Applicant: Novozymes A/S

Inventor： Debbie Yaver ,  Astrid Mach-Aigner ,  Robert Mach ,  Petra Till

IPC: C12N9/02 ,  C12N15/113 ,  C12N15/80

CPC classification number: C12N9/0061 ,  C12N15/1137 ,  C12Y302/01004 ,  C12N15/80 ,  C12N2800/10

Abstract: The instant invention relates to a filamentous fungal host cell producing a polypeptide of interest, said host cell comprising one or more native or heterologous polynucleotide encoding a long non-coding RNA (lncRNA), wherein said lncRNA comprises more than 262 nucleotides and comprises a contiguous nucleotide sequence at its 3′ end, absent any poly-adenylation tail, that is at least 70% identical to that of SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO: 10, SEQ ID NO: 19, SEQ ID NO:20 or SEQ ID NO:21; and/or said lncRNA comprises 2 or more Xyr1 binding sequences, as well as methods of producing a polypeptide of interest in said host cells and methods of improving the production of a polypeptide of interest.