公开(公告)号：US20220025423A1

公开(公告)日：2022-01-27

申请号：US17296938

申请日：2019-11-26

Applicant: Novozymes A/S

Inventor： Nicholas Jochumsen ,  Michael Rey ,  Chiho Inoue

IPC: C12P21/02 ,  C12N9/04 ,  C12N15/80

Abstract: The present invention relates to mutated filamentous fungal host cell producing a secreted polypeptide of interest, wherein a native putative steroid dehydrogenase is modified, truncated, partly or fully inactivated, present at reduced level or eliminated compared to a non-mutated parent cell, and wherein said native putative steroid dehydrogenase comprises at least one conserved amino acid motif selected from: YGAR and/or VPHS[W/Y]F and/or QC[A/V/S]RRL and/or LKKYTLP and/or CPHYT, and methods of producing a secreted polypeptide of interest in said cells as well as methods of producing said cells.