公开(公告)号：US20190352709A1

公开(公告)日：2019-11-21

申请号：US16465987

申请日：2017-11-29

申请人： Oxford Nanopore Technologies Limited

发明人： James Clarke ,  James White ,  Richard Muscat ,  Jessica Mary May Knott ,  Ramiz Iqbal Nathani ,  Andrew John Heron ,  Mark John Bruce ,  Lakmal Nishantha Jayasinghe ,  Domenico Caprotti ,  David Jackson Stoddart ,  Rebecca Victoria Bowen ,  Christopher James Wright ,  Paul Richard Moody

IPC分类号： C12Q1/6869 ,  G01N33/487

摘要： Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.