公开(公告)号：US20180371256A1

公开(公告)日：2018-12-27

申请号：US15955877

申请日：2018-04-18

Applicant: POSTECH ACADEMY-INDUSTRY FOUNDATION

Inventor： Kyo Han Ahn ,  Yong Woong Jun ,  Hyerim Kim ,  Subhankar Singha ,  Ye Jin Reo

IPC: C09B57/02 ,  G01N33/58 ,  C09K11/06

Abstract: Provided is a method for preparing a novel benzocoumarin-based two-photon absorbing fluorescent dye, such as a pyridinium-benzocoumarin (Py+BC) derivative compound and its precursor compound (pyridyl-benzocoumarin, PyBC), an aryl-benzocoumarin (ArBC) derivative compound, a benzothiazolyl-benzocoumarin (BtBC) derivative compound or a keto-benzocoumarin (KetoBC) derivative compound. The Py+BC derivative compound of the present invention is a two-photon absorbing near-infrared fluorescent dye, which may minimize interference of autofluorescence compared to conventional two-photon absorbing fluorescent dyes such as acedan and naphthalimide, thereby obtaining a high-definition image, and therefore is expected to be effectively used in imaging studies. In addition, when the two-photon absorbing near-infrared fluorescent dye of the present invention is used, it is expected to be suitable for high resolution imaging of deep tissue.