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公开(公告)号:US20200248255A1
公开(公告)日:2020-08-06
申请号:US16782044
申请日:2020-02-04
申请人: PRESIDENT AND FELLOWS OF HARVARD COLLEGE , THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人: Jason Daniel Buenrostro , Sai Ma , Aviv Regev
IPC分类号: C12Q1/6874 , C12N15/10 , C12Q1/6853
摘要: Provided herein include methods and compositions for analyzing nucleic acid in individual cells. In some embodiments, the methods herein include generating, within individual cells, fragmented cellular genomic DNA and cDNA copies of cellular RNA molecules, barcoding the fragmented genomic DNA and the cDNA within each cell such that the genomic DNA and the cDNA from the same cell receive the same unique barcode sequence, isolating the barcoded genomic DNA and cDNA, and characterizing one or more features of the individual cells based, at least in part, on sequencing of the isolated barcoded genomic DNA and the cDNA.
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公开(公告)号:US11634766B2
公开(公告)日:2023-04-25
申请号:US16782044
申请日:2020-02-04
申请人: PRESIDENT AND FELLOWS OF HARVARD COLLEGE , THE BROAD INSTITUTE, INC. , MASSACHUSETTS INSTITUTE OF TECHNOLOGY
发明人: Jason Daniel Buenrostro , Sai Ma , Aviv Regev
IPC分类号: C12Q1/68 , C12Q1/6874 , C12N15/10 , C12Q1/6853
摘要: Provided herein include methods and compositions for analyzing nucleic acid in individual cells. In some embodiments, the methods herein include generating, within individual cells, fragmented cellular genomic DNA and cDNA copies of cellular RNA molecules, barcoding the fragmented genomic DNA and the cDNA within each cell such that the genomic DNA and the cDNA from the same cell receive the same unique barcode sequence, isolating the barcoded genomic DNA and cDNA, and characterizing one or more features of the individual cells based, at least in part, on sequencing of the isolated barcoded genomic DNA and the cDNA.
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