公开(公告)号：US11447823B2

公开(公告)日：2022-09-20

申请号：US16844797

申请日：2020-04-09

Applicant: Pacific Biosciences of California, Inc.

Inventor： Sean Stromberg ,  John Vieceli ,  Kandaswamy Vijayan ,  Arnold Oliphant

IPC: C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6853 ,  C12Q1/6832

Abstract: A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.

公开(公告)号：US12180545B2

公开(公告)日：2024-12-31

申请号：US17876893

申请日：2022-07-29

Applicant: Pacific Biosciences of California, Inc.

Inventor： Sean Stromberg ,  John Vieceli ,  Kandaswamy Vijayan ,  Arnold Oliphant

IPC: C12Q1/68 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874

Abstract: A method of determining a nucleic acid sequence that includes steps of: (a) contacting a primed template nucleic acid with a series of mixtures for forming ternary complexes, wherein each of the mixtures includes a polymerase and nucleotide cognates for at least two different base types suspected of being present at the next template position of the template nucleic acid; (b) monitoring the next template position for ternary complexes formed by the series of mixtures, wherein a signal state indicates presence or absence of ternary complex formed at the next template position by each individual mixture, thereby determining a series of signal states that encodes a base call for the next template position; and (c) decoding the series of signal states to distinguish a correct base call for the next template position from an error in the base call.