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    MUTAGENESIS METHOD USING POLYETHYLENE GLYCOL MEDIATED INTRODUCTION OF MUTAGENIC NUCLEOBASES INTO PLANT PROTOPLASTS
    1.
    发明申请
    MUTAGENESIS METHOD USING POLYETHYLENE GLYCOL MEDIATED INTRODUCTION OF MUTAGENIC NUCLEOBASES INTO PLANT PROTOPLASTS 有权
    使用聚乙二醇将诱导性核酸引入植物原核生物的诱变方法

    公开(公告)号：US20120282699A1

    公开(公告)日：2012-11-08

    申请号：US13551143

    申请日：2012-07-17

    申请人： Paul BUNDOCK ,  Michiel Theodoor Jan De Both ,  Franck Lhuissier

    发明人： Paul BUNDOCK ,  Michiel Theodoor Jan De Both ,  Franck Lhuissier

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8206 ,  C12N15/8213

    摘要： Method for targeted alteration of a duplex acceptor DNA sequence in a plant cell protoplast, comprising combining the duplex acceptor DNA sequence with a donor mutagenic nucleobase, wherein the duplex acceptor DNA sequence contains a first DNA sequence and a second DNA sequence which is the complement of the first DNA sequence and wherein the donor mutagenic nucleobase comprises at least one mismatch with respect to the duplex acceptor DNA sequence to be altered, preferably with respect to the first DNA sequence, wherein the method further comprises a step of introducing the donor mutagenic nucleobase into the cell protoplasts using polyethylene glycol (PEG) mediated transformation and the use of PEG protoplast transformation for enhancing the rate of targeted mutagenesis.

    摘要翻译： 用于靶向改变植物细胞原生质体中双链体受体DNA序列的方法，包括将双链体受体DNA序列与供体诱变核碱基组合，其中双链体受体DNA序列含有第一DNA序列和第二DNA序列， 所述第一DNA序列，并且其中所述供体诱变核碱基相对于待改变的双链体受体DNA序列至少包含一个错配，优选相对于所述第一DNA序列，其中所述方法还包括将供体诱变核碱基引入 使用聚乙二醇（PEG）介导的转化的细胞原生质体和使用PEG原生质体转化来提高定向诱变的速率。