公开(公告)号：US11293015B2

公开(公告)日：2022-04-05

申请号：US16951916

申请日：2020-11-18

申请人： Peking University ,  Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College

发明人： Xiaoguang Lei ,  Jungui Dai ,  Lei Gao ,  Cong Su

IPC分类号： C12N9/50 ,  C12P7/26 ,  C12P17/04

摘要： The present invention provides a Diels-Alderase and use thereof, and belongs to the field of gene engineering technology. The Diels-Alderase is MaDA, and its amino acid sequence and gene sequence are represented by SEQ ID Nos. 1 and 2, respectively. The present invention also provides MaDA-1 and MaDA-2, both of which are homologous proteins of MaDA, and their amino acid sequences are represented by SEQ ID Nos. 10 and 12, respectively. The present invention has discovered that MaDA and its homologous proteins from Morus alba can stereospecifically synthesize natural products of endo configuration, and prepare D-A type natural products and their analogs in vitro using chalcones and dehydroprenyl-containing compounds as substrates, which helps to develop and utilize the medicinal value of such natural products, and also provides a possibility to synthesize other six-membered ring-containing important chemical precursors or natural products.