公开(公告)号：US20120101004A1

公开(公告)日：2012-04-26

申请号：US13377121

申请日：2010-06-09

申请人： Peter Garred ,  Christian Honoré

发明人： Peter Garred ,  Christian Honoré

IPC分类号： C40B30/04 ,  G01N33/566

CPC分类号： G01N33/564 ,  G01N2333/4716

摘要： The present invention relates to methods for detecting Ficolin-3 dependent activation of the lectin pathway of complement, methods for identifying abnormalities in Ficolin-3, and methods for screening for deficiencies/and or identifying abnormalities in any downstream components of the Ficolin-3 dependent activation of the lectin pathway of 5 complement using an acetylated Ficolin-3 ligand, said methods generally comprising the steps of: (a) providing a sample of blood, serum, plasma, another body fluid or an extract thereof; (b) (optionally) preventing in the sample activation of the classical pathway and/or the alternative pathway and/or any non-Ficolin-3 mediated activation of the lectin pathway; (c) acetylating a molecule; (d) contacting said acetylated molecule 10 with said sample, in conditions that permit specific binding of Ficolin-3 to said acetylated molecule, and, (e) detecting and quantifying specific binding of the Ficolin-3 to said acetylated molecule, (f) determining in the sample complement activation and/or deposition by the detection of a C2, C3, C4 and/or a C5 cleavage product and/or by detecting any of the terminal complement complex components C6, C7, C8 or C9 or 15 the C5b-9 terminal complement complex as such. The present invention also provides assays and kits comprising the methods of the invention.

摘要翻译： 本发明涉及用于检测补体的凝集素途径的Ficolin-3依赖性活化的方法，用于鉴定Ficolin-3异常的方法，以及筛选Ficolin-3依赖性的任何下游组分的缺陷和/或鉴定异常的方法 所述方法通常包括以下步骤：（a）提供血液，血清，血浆，另一体液或其提取物的样品;（b）使用乙酰化的Ficolin-3配体激活5个补体的凝集素途径。 （b）（任选地）阻止样品激活经典途径和/或替代途径和/或任何非Ficolin-3介导的凝集素途径的活化; （c）乙酰化分子; （d）使所述乙酰化分子10与所述样品接触，条件是允许Ficolin-3与所述乙酰化分子的特异性结合，和（e）检测和定量Ficolin-3与所述乙酰化分子的特异性结合，（f） 通过检测C2，C3，C4和/或C5切割产物和/或通过检测任何终末互补复合物组分C6，C7，C8或C9或15来确定样品中补体活化和/或沉积C5b -9末端补体复合物。 本发明还提供包含本发明方法的测定和试剂盒。