公开(公告)号：US5624808A

公开(公告)日：1997-04-29

申请号：US412256

申请日：1995-03-28

申请人： Peter Thompson ,  Fridjtof Lund-Johannsen

发明人： Peter Thompson ,  Fridjtof Lund-Johannsen

IPC分类号： G01N33/569 ,  G01N33/68 ,  G01N33/53 ,  G01N33/536 ,  G01N33/574

CPC分类号： G01N33/56966 ,  G01N33/68 ,  Y10S435/96 ,  Y10S435/968 ,  Y10S436/80 ,  Y10S436/813 ,  Y10S436/824 ,  Y10T436/25125

摘要： A novel approach to study changes in protein tyrosine phosphorylation during apoptosis, and thereby identify cells committed to apoptosis is presented, methods used to study apoptosis and tyrosine phosphorylation at the single cell level are combined to study directly whether apoptosis in hematopoietic cells is associated with changes in cellular phosphotyrosine levels. The changes in cellular phosphotyrosine content strongly correlated with the appearance of features of cell death such as cell shrinkage, DNA fragmentation and loss of membrane integrity.

摘要翻译： 研究了凋亡过程中研究蛋白酪氨酸磷酸化变化的新方法，从而鉴定了致死细胞凋亡的细胞，结合单细胞水平研究细胞凋亡和酪氨酸磷酸化的方法，直接研究造血细胞凋亡与变化相关 在细胞磷酸酪氨酸水平。 细胞磷酸酪氨酸含量的变化与细胞死亡特征的出现密切相关，如细胞收缩，DNA断裂和膜完整性丧失。