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1.发明授权Methods for the preservation of cells and tissues using trimethylamine oxide or betaine with raffinose or trehalose 失效使用三甲胺氧化物或甜菜碱与棉子糖或海藻糖保存细胞和组织的方法
公开(公告)号：US5827640A
公开(公告)日：1998-10-27
申请号：US722306
申请日：1996-09-30
CPC分类号： A01N1/02 , A01N1/0221 , C12N1/04
摘要： The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. The solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3M, and a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties. The inventive solutions preferably contain CaSO.sub.4, together with combinations of anions and cations from the protein-stabilizing ends of the Hofmeister series, such as K.sub.2 SO.sub.4. In particular methods using solutions comprising raffinose or trehalose and trimethyl amine oxide or betaine, wherein the solution is substantially isotonic with the biological material and the solution is substantially free of iodide, dihydrogen phosphate, bicarbonate, nitrate and bisulfate are described.
摘要翻译： 本发明提供了用于保存生物材料的方法和方法，所述生物材料能够使器官，组织和细胞长时间储存，同时生物活性损失最小。 本发明的溶液与待保存的生物材料基本上是等渗的，并且基本上不含一价的氧阴离子和碘化物。 溶液包含分子量至少为约335且在水中的溶解度至少为约0.3M的第一中性溶质和分子量小于约200的第二中性溶质，并具有亲水和疏水部分。 本发明的溶液优选含有CaSO 4，以及来自Hofmeister系列的蛋白质稳定末端的阴离子和阳离子的组合，例如K 2 SO 4。 特别是使用包含棉子糖或海藻糖和三甲基氧化胺或甜菜碱的溶液的方法，其中所述溶液与生物材料基本上是等渗的，并且溶液基本上不含碘化物，磷酸二氢盐，碳酸氢盐，硝酸盐和硫酸氢盐。
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公开(公告)号：US06361933B1
公开(公告)日：2002-03-26
申请号：US09512139
申请日：2000-02-23
IPC分类号： A01N100
CPC分类号： A01N1/02 , A01N1/0221 , A01N1/0226 , C12N1/04 , Y10T436/108331
摘要： The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate, bisulfate and iodide. In one embodiment, preferred for the preservation of platelets, the solutions comprise betaine, sodium chloride and sodium citrate. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.
摘要翻译： 本发明提供了用于保存活体生物材料的方法和方法，其使器官，组织和细胞能够以最小的生物活性损失被长时间储存。 本发明溶液与待保存的生物材料基本上是等渗的，并且基本上不含磷酸二氢盐，碳酸氢盐，硝酸盐，硫酸氢盐和碘化物。 在一个实施方案中，优选用于保存血小板，溶液包括甜菜碱，氯化钠和柠檬酸钠。 为了保存许多活的生物材料，本发明的溶液优选含有选自硫酸钙和氯化钙的钙盐。
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3.发明授权Compositions comprising betaine, sodium citrate and sodium chloride and methods for the preservation of biological materials 失效包含甜菜碱，柠檬酸钠和氯化钠的组合物以及保存生物材料的方法
公开(公告)号：US6037116A
公开(公告)日：2000-03-14
申请号：US85318
申请日：1998-05-26
CPC分类号： A01N1/0226 , A01N1/02 , A01N1/0221 , C12N1/04
摘要： The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate, bisulfate and iodide. In one embodiment preferred for the preservation of platelets, the solutions comprise betaine, sodium chloride and sodium citrate. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.
摘要翻译： 本发明提供了用于保存活体生物材料的方法和方法，其使器官，组织和细胞能够以最小的生物活性损失被长时间储存。 本发明溶液与待保存的生物材料基本上是等渗的，并且基本上不含磷酸二氢盐，碳酸氢盐，硝酸盐，硫酸氢盐和碘化物。 在优选用于保存血小板的一个实施方案中，溶液包含甜菜碱，氯化钠和柠檬酸钠。 为了保存许多活的生物材料，本发明的溶液优选含有选自硫酸钙和氯化钙的钙盐。
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公开(公告)号：US5962213A
公开(公告)日：1999-10-05
申请号：US989470
申请日：1997-12-12
CPC分类号： A01N1/0226 , A01N1/02 , A01N1/0221 , C12N1/04
摘要： The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate bisulfate and iodide. In one embodiment, the solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3 M, a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties, with the first neutral solute preferably being selected from the group consisting of raffinose and trehalose and the second neutral solute preferably being selected from the group consisting of trimethyl amine oxide and betaine. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.
摘要翻译： 本发明提供了用于保存活体生物材料的方法和方法，其使器官，组织和细胞能够以最小的生物活性损失被长时间储存。 本发明溶液与待保存的生物材料基本上是等渗的，并且基本上不含磷酸二氢盐，碳酸氢盐，硝酸盐硫酸氢盐和碘化物。 在一个实施方案中，溶液包含具有至少约335分子量和至少约0.3M的在水中的溶解度的第一中性溶质，分子量小于约200的第二中性溶质，并且具有亲水性和 疏水部分，第一中性溶质优选选自棉子糖和海藻糖，第二中性溶质优选选自三甲胺氧化物和甜菜碱。 为了保存许多活的生物材料，本发明的溶液优选含有选自硫酸钙和氯化钙的钙盐。
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公开(公告)号：US5879875A
公开(公告)日：1999-03-09
申请号：US662244
申请日：1996-06-14
CPC分类号： A01N1/02 , A01N1/0221 , C12N1/04
摘要： The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. The solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3M, and a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties. The inventive solutions preferably contain CaSO.sub.4, together with combinations of anions and cations from the protein-stabilizing ends of the Hofmeister series, such as K.sub.2 SO.sub.4. Solutions with raffinose or trehalose in combination with trimethyl amine oxide or betaine are particularly preferred.
摘要翻译： 本发明提供了用于保存生物材料的方法和方法，所述生物材料能够使器官，组织和细胞长时间储存，同时生物活性损失最小。 本发明的溶液与待保存的生物材料基本上是等渗的，并且基本上不含一价的氧阴离子和碘化物。 溶液包含分子量至少为约335且在水中的溶解度至少为约0.3M的第一中性溶质和分子量小于约200的第二中性溶质，并具有亲水和疏水部分。 本发明的溶液优选含有CaSO 4，以及来自Hofmeister系列的蛋白质稳定末端的阴离子和阳离子的组合，例如K 2 SO 4。 特别优选棉子糖或海藻糖与三甲基氧化胺或甜菜碱组合的溶液。
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6.发明授权Composition comprising raffinose, TMAO, sodium citrate and methods for the preservation of living tissues 失效包含棉子糖，TMAO，柠檬酸钠的组合物和用于保存活组织的方法
公开(公告)号：US6114107A
公开(公告)日：2000-09-05
申请号：US842553
申请日：1997-04-15
CPC分类号： C12N1/04 , A01N1/02 , A01N1/0221
摘要： The present invention provides solutions and methods for preserving biological material that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of univalent oxyanions and of iodide. In one embodiment, the solutions comprise a first neutral solute having a molecular weight of at least about 335 and a solubility in water of at least about 0.3 M, a second neutral solute having a molecular weight of less than about 200 and having both hydrophilic and hydrophobic moieties, and sodium citrate. The inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.
摘要翻译： 本发明提供了用于保存生物材料的方法和方法，所述生物材料能够使器官，组织和细胞长时间储存，同时生物活性损失最小。 本发明的溶液与待保存的生物材料基本上是等渗的，并且基本上不含一价的氧阴离子和碘化物。 在一个实施方案中，溶液包含具有至少约335分子量和至少约0.3M的在水中的溶解度的第一中性溶质，分子量小于约200的第二中性溶质，并且具有亲水性和 疏水部分和柠檬酸钠。 本发明的溶液优选含有选自硫酸钙和氯化钙的钙盐。
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公开(公告)号：US06743575B2
公开(公告)日：2004-06-01
申请号：US10096635
申请日：2002-03-12
IPC分类号： A01N102
CPC分类号： C12N1/04 , A01N1/02 , A01N1/0221
摘要： The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of biological activity. The inventive solutions are substantially isotonic with the biological material to be preserved and are substantially free of dihydrogen phosphate, bicarbonate, nitrate, bisulfate and iodide. In one embodiment, preferred for the preservation of platelets, the solutions comprise betaine, sodium chloride and sodium citrate. For the preservation of many living biological materials, the inventive solutions preferably contain a calcium salt selected from the group consisting of calcium sulfate and calcium chloride.
摘要翻译： 本发明提供了用于保存活体生物材料的方法和方法，其使器官，组织和细胞能够以最小的生物活性损失被长时间储存。 本发明溶液与待保存的生物材料基本上是等渗的，并且基本上不含磷酸二氢盐，碳酸氢盐，硝酸盐，硫酸氢盐和碘化物。 在一个实施方案中，优选用于保存血小板，溶液包括甜菜碱，氯化钠和柠檬酸钠。 为了保存许多活的生物材料，本发明的溶液优选含有选自硫酸钙和氯化钙的钙盐。
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