公开(公告)号：US07087433B2

公开(公告)日：2006-08-08

申请号：US10227367

申请日：2002-08-25

申请人： Qiang Zhou ,  Zhiyuan Yang ,  Qingwei Zhu ,  Kunxin Luo

发明人： Qiang Zhou ,  Zhiyuan Yang ,  Qingwei Zhu ,  Kunxin Luo

IPC分类号： A61K48/00 ,  C07H21/04 ,  C07H21/02 ,  C12Q1/68

CPC分类号： C12N15/113

摘要： A method for altering transcription in a cell comprising an amount of active CDK9/cyclin, comprises the steps of: (a) introducing in the cell an agent which modulates the amount of active CDK9/cyclin in the cell, and thereby alters transcription in the cell, wherein the agent comprises an RNA selected from the group consisting of an RNA aptamer that specifically binds CDK9/cyclin, a CDK9/cyclin-binding domain of 7SK RNA, a 7SK RNA-binding antisense 7SK RNA domain, and a 7SK RNA-specific RNAi, and (b) detecting a resultant altering of transcription in the cell.Methods for screening for an agent which modulates 7SK RNA-CDK9/cyclin binding generally comprise the steps of (a) incubating a mixture of 7SK RNA, CDK9/cyclin and a candidate agent under conditions wherein but for the presence of the agent, the 7SK RNA and CDK9/cyclin engage in a reference binding; and (b) detecting an agent-biased binding of the 7SK RNA to the CDK9/cyclin.

摘要翻译： 一种用于在包含一定量活性CDK9 /细胞周期蛋白的细胞中改变转录的方法包括以下步骤：（a）在细胞中引入调节细胞中活性CDK9 /细胞周期蛋白的量的试剂，从而改变细胞内的转录 细胞，其中所述试剂包含选自特异性结合CDK9 /细胞周期蛋白的RNA适配体，7SK RNA的CDK9 /细胞周期蛋白结合结构域，7SK RNA结合反义7SK RNA结构域和7SK RNA- 特异性RNAi，和（b）检测所得到的细胞转录的变化。 用于筛选调节7SK RNA-CDK9 /细胞周期蛋白结合的试剂的方法通常包括以下步骤：（a）在7SK RNA，CDK9 /细胞周期蛋白和候选试剂的混合物的条件下， RNA和CDK9 /细胞周期蛋白参与参考结合; 和（b）检测7SK RNA与CDK9 /细胞周期蛋白的药剂偏置结合。

公开(公告)号：US20060239976A1

公开(公告)日：2006-10-26

申请号：US11481190

申请日：2006-07-01

申请人： Qiang Zheu ,  Zhiyuan Yang ,  Qingwei Zhu ,  Kunxin Luo

发明人： Qiang Zheu ,  Zhiyuan Yang ,  Qingwei Zhu ,  Kunxin Luo

IPC分类号： A61K48/00 ,  C12N15/867

CPC分类号： C12N15/113

摘要： A method for altering transcription in a cell comprising an amount of active CDK9/cyclin, comprises the steps of: (a) introducing in the cell an agent which modulates the amount of active CDK9/cyclin in the cell, and thereby alters transcription in the cell, wherein the agent comprises an RNA selected from the group consisting of an RNA aptamer that specifically binds CDK9/cyclin, a CDK9/cyclin-binding domain of 7SK RNA, a 7SK RNA-binding antisense 7SK RNA domain, and a 7SK RNA-specific RNAi, and (b) detecting a resultant altering of transcription in the cell. Methods for screening for an agent which modulates 7SK RNA-CDK9/cyclin binding generally comprise the steps of (a) incubating a mixture of 7SK RNA, CDK9/cyclin and a candidate agent under conditions wherein but for the presence of the agent, the 7SK RNA and CDK9/cyclin engage in a reference binding; and (b) detecting an agent-biased binding of the 7SK RNA to the CDK9/cyclin.

摘要翻译： 一种用于在包含一定量活性CDK9 /细胞周期蛋白的细胞中改变转录的方法包括以下步骤：（a）在细胞中引入调节细胞中活性CDK9 /细胞周期蛋白的量的试剂，从而改变细胞内的转录 细胞，其中所述试剂包含选自特异性结合CDK9 /细胞周期蛋白的RNA适配体，7SK RNA的CDK9 /细胞周期蛋白结合结构域，7SK RNA结合反义7SK RNA结构域和7SK RNA- 特异性RNAi，和（b）检测所得到的细胞转录的变化。 用于筛选调节7SK RNA-CDK9 /细胞周期蛋白结合的试剂的方法通常包括以下步骤：（a）在7SK RNA，CDK9 /细胞周期蛋白和候选试剂的混合物的条件下， RNA和CDK9 /细胞周期蛋白参与参考结合; 和（b）检测7SK RNA与CDK9 /细胞周期蛋白的药剂偏置结合。