公开(公告)号：US4967825A

公开(公告)日：1990-11-06

申请号：US216762

申请日：1988-07-08

申请人： R. Malcolm Brown, Jr. ,  Shun Mizuta

发明人： R. Malcolm Brown, Jr. ,  Shun Mizuta

IPC分类号： B22D23/00

CPC分类号： B82Y30/00 ,  B22D23/00 ,  Y10S977/881 ,  Y10S977/89 ,  Y10S977/902

摘要： Disclosed are improved methods for preparing high resolution replicas for electron microscopic analysis. Replicas are prepared having a resolution capability less than ten angstroms, with structures of even less than five angstroms being readily discernible. Particular aspects of the invention concern the use of a platinum/carbon evaporant to prepare extremely thin replica surfaces which allow the visualization of structures heretofore unresolvable. The replication is performed at high vacuum, on the order of 10.sup.-5 Torr or better, with continuous rotation of the sample to allow for an even distribution of the evaporant. The resultant replica surfaces are on the order of no more than 5 to 10 angstroms thick. The replica technology disclosed herein is applicable to both biological as well as non-biological samples, including tissues, biochemicals, metals and polymers and even computer chips and superconductor surfaces.

摘要翻译： 公开了用于制备用于电子显微镜分析的高分辨率复制品的改进方法。 准备了具有小于10埃的分辨能力的复制品，其结构甚至小于5埃是易于识别的。 本发明的特定方面涉及使用铂/碳蒸发器来制备极薄的复制表面，其允许可视化迄今为止尚未解决的结构。 复制在高真空下进行，大约为10 -5 Torr或更好，样品连续旋转以允许蒸发剂的均匀分布。 所得复制表面的厚度不超过5至10埃。 本文公开的复制技术适用于生物和非生物样品，包括组织，生物化学物质，金属和聚合物，甚至计算机芯片和超导体表面。