公开(公告)号：US20130217039A1

公开(公告)日：2013-08-22

申请号：US13704708

申请日：2011-06-20

申请人： R. Steven Brown ,  Eric Marcotte ,  Michael Miron ,  Tom Radcliffe

发明人： R. Steven Brown ,  Eric Marcotte ,  Michael Miron ,  Tom Radcliffe

IPC分类号： G01N21/64

CPC分类号： G01N21/6486 ,  G01N21/274 ,  G01N21/6452 ,  G01N33/582 ,  G01N2021/6484 ,  G01N2201/062

摘要： A system for detecting presence of an organism having an enzyme in a sample, comprising: a cartridge for containing the sample and a substrate such that the enzyme can react with the substrate to produce a biological molecule; a partitioning element mounted in a recess in a base of the cartridge, the partitioning element allowing partitioning of the biological molecule thereinto; a light source for irradiating the biological molecule partitioned into the partitioning element; and, a detector for detecting fluorescence of the biological molecule partitioned into the partitioning element, the detected fluorescence being indicative of presence of the organism in the sample; wherein the light source is in a raised cartridge mount of the system that mates with the recess in the base of the cartridge. Also provided is a method for calibrating said system and quality control cartridges for use in same.

摘要翻译： 一种用于检测样品中具有酶的生物体的存在的系统，包括：用于容纳样品和底物的筒，使得酶可以与底物反应以产生生物分子; 安装在所述盒的基部中的凹部中的分隔元件，所述分隔元件允许生物分子在其中分配; 用于照射被划分成分隔元件的生物分子的光源; 以及检测器，用于检测划分成分隔元件的生物分子的荧光，检测到的荧光指示样品中生物体的存在; 其中所述光源位于所述系统的凸起的盒安装件中，以与所述盒的所述底座中的凹部配合。 还提供了用于校准所述系统和用于其的质量控制盒的方法。