公开(公告)号：US20240344088A1

公开(公告)日：2024-10-17

申请号：US18750678

申请日：2024-06-21

Applicant: RAMOT AT TEL-AVIV UNIVERSITY LTD.

Inventor： Adi BARZEL ,  Iris DOTAN ,  Carmel PUNDAK-MINTZ ,  Daniel NATAF ,  Miriam FRIED ,  Natalia GRITSENKO

IPC: C12N15/90 ,  C07K16/28 ,  C12N15/86

CPC classification number: C12N15/907 ,  C07K16/2809 ,  C12N15/86 ,  C07K2317/14 ,  C07K2317/24 ,  C07K2317/76 ,  C12N2510/00

Abstract: The present invention relates to methods for targeted insertion of at least one nucleic acid sequence/s of interest into a target genomic locus of a mammalian cell. More specifically, the methods of the invention are based on using nucleic acid cassettes comprising the nucleic acid sequence/s of interest and at least one recognition signal sequence (RSS), for insertion of the nucleic acid sequence of interest into the target genomic locus that is mediated by RAG-catalyzed recombination. The invention further provides cassettes, vectors and vehicles and cells comprising said cassettes, compositions and uses thereof in immunotherapy.