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公开(公告)号：US20200277571A1
公开(公告)日：2020-09-03
申请号：US16647454
申请日：2018-09-14
Inventor： Daiki NUKAYA , Mototsugu EIRAKU , Yuiko KINOSE , Akishi ONISHI , Masayo TAKAHASHI , Yoshiki SASAI (deceased)
Abstract: The present invention aims to provide a retinal tissue rich in cone photoreceptor precursors and/or cone photoreceptors, a retinal tissue rich in rod photoreceptor precursors and/or rod photoreceptors, and a production method thereof and the like. i) A method for increasing a proportion of a cone photoreceptor precursor and a cone photoreceptor in a photoreceptor precursor and a photoreceptor contained in a retinal tissue, including a step of culturing a retinal tissue, in an initial developmental stage to a stage where an emergence rate of a cone photoreceptor precursor reaches maximum, in a medium containing a dorsalization signal transmitter at a concentration sufficient to suppress expression of a ventral marker, or ii) a method for increasing a proportion of a rod photoreceptor precursor and a rod photoreceptor in a photoreceptor precursor and a photoreceptor contained in a retinal tissue, including a step of culturing a retinal tissue, in an initial developmental stage to a stage where an emergence rate of a cone photoreceptor precursor reaches maximum, in a medium containing a ventralization signal transmitter at a concentration sufficient to promote expression of a ventral marker.
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公开(公告)号：US20220249698A1
公开(公告)日：2022-08-11
申请号：US17626042
申请日：2020-07-10
Applicant: RIKEN
Inventor： Masayo TAKAHASHI , Akishi ONISHI , Yuji TSUNEKAWA
IPC: A61K48/00 , A61K38/46 , A61K31/7088 , A61K38/17 , A61P27/02
Abstract: Provided is a novel therapeutic agent for a disease caused by a dominant gene mutation (7). A therapeutic agent of the present invention comprises a donor DNA (20) that contains a polynucleotide having the following sequences (a) to (c): (a) a normal gene (1); (b) a first reverse target sequence (2a) that is located upstream of the normal gene (1) and that is cleaved by a designer nuclease; and (c) a second reverse target sequence (2b) that is located downstream of the normal gene (1) and that is cleaved by the designer nuclease, where the reverse target sequences (2a, 2b) each mean a sequence obtained by inverting a target sequence (6) that is present in the genome and that is cleaved by the designer nuclease.
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公开(公告)号：US20240399026A1
公开(公告)日：2024-12-05
申请号：US18805131
申请日：2024-08-14
Applicant: RIKEN , SUMITOMO PHARMA CO., LTD.
Inventor： Daiki NUKAYA , Mototsugu EIRAKU , Yuiko KINOSE , Akishi ONISHI , Masayo TAKAHASHI , Yoshiki SASAI (deceased)
IPC: A61L27/38 , C12N5/0793 , G01N33/50
Abstract: The present invention aims to provide a method for suppressing differentiation of ganglion cell, amacrine cell, horizontal cell and/or bipolar cell in a neural retina tissue containing photoreceptor precursor and/or photoreceptor, and the like. A method for suppressing differentiation of a ganglion cell, an amacrine cell, a horizontal cell and/or a bipolar cell in a neural retinal tissue containing a photoreceptor precursor and/or a photoreceptor, including a step of culturing a retinal tissue comprising a neural retinal progenitor cell and in any stage between a differentiation stage immediately after emergence of a ganglion cell and a differentiation stage where emergence rate of a cone photoreceptor precursor reaches maximum in a medium containing a thyroid gland hormone signal transduction pathway agonist.
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公开(公告)号：US20210154370A1
公开(公告)日：2021-05-27
申请号：US16647441
申请日：2018-09-14
Inventor： Daiki NUKAYA , Mototsugu EIRAKU , Yuiko KINOSE , Akishi ONISHI , Masayo TAKAHASHI , Yoshiki SASAI (deceased)
IPC: A61L27/38 , C12N5/0793 , G01N33/50
Abstract: The present invention aims to provide a method for suppressing differentiation of ganglion cell, amacrine cell, horizontal cell and/or bipolar cell in a neural retina tissue containing photoreceptor precursor and/or photoreceptor, and the like. A method for suppressing differentiation of a ganglion cell, an amacrine cell, a horizontal cell and/or a bipolar cell in a neural retinal tissue containing a photoreceptor precursor and/or a photoreceptor, including a step of culturing a retinal tissue comprising a neural retinal progenitor cell and in any stage between a differentiation stage immediately after emergence of a ganglion cell and a differentiation stage where emergence rate of a cone photoreceptor precursor reaches maximum in a medium containing a thyroid gland hormone signal transduction pathway agonist.
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