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公开(公告)号:US20230020428A1
公开(公告)日:2023-01-19
申请号:US17862254
申请日:2022-07-11
发明人: Jonathan Wert , Li Zhi , Dingjiang Liu
IPC分类号: G01N33/569 , G01N33/53 , G01N30/96
摘要: Methods for determining the relative abundance of intact adeno-associated virus (AAV) capsid components in a sample of recombinant AAV particles are disclosed. In embodiments, the methods include a system regeneration process that minimizes or eliminates the presence of ghost peaks to maximize analytical accuracy and ensure product quality and consistency.
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公开(公告)号:US20230016717A1
公开(公告)日:2023-01-19
申请号:US17862240
申请日:2022-07-11
发明人: Haibo Qiu , Zhijie Wu , Hongxia Wang , Ning Li , Jonathan Wert
摘要: Methods for identifying viral protein constituents and quantifying the relative abundance of such viral protein constituents in a sample of viral particles are disclosed. In embodiments, the methods include first-dimension chromatography to separate intact viral capsid components of the sample, online denaturation of the viral capsid components to produce intact viral proteins, second-dimension chromatography to separate the viral proteins, and mass spectrometry to determine the masses of the viral proteins and identify the viral protein constituents of the sample.
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