公开(公告)号：US20130034879A1

公开(公告)日：2013-02-07

申请号：US13564940

申请日：2012-08-02

申请人： Remigijus Skirgaila ,  Agne Tubeleviciute ,  Renata Rimseliéné ,  Sigitas Burinskas

发明人： Remigijus Skirgaila ,  Agne Tubeleviciute ,  Renata Rimseliéné ,  Sigitas Burinskas

IPC分类号： C12N9/12 ,  C40B10/00 ,  C12P19/34

CPC分类号： C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

摘要： A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.

摘要翻译： 一种DNA聚合酶突变体，其包含在一个或多个以下选择的氨基酸位置具有突变的Taq DNA聚合酶氨基酸序列：E189K，E230K，E507K，H28R，L30R，G38R，F73V，H75R，E76A，E76G，E76K，E90K ，K206R，E315K，A348V，L351F，A439T，D452N，G504S，E507A，D551N，L552R，I553V，D578N，H676R，Q680R，D732G，E734G，E734K，F749V; 其中所述聚合酶突变体相对于野生型DNA聚合酶显示出增加的聚合酶速度，对DNA底物的增加的亲和力和/或对DNA聚合酶抑制剂的增加的抗性; 并且其中当所述突变与两个或更多个其它突变组合时为E507K时，或者突变为Q680R与四个或更多个其它突变组合时，至少一个其它突变位于选定氨基酸位置之一; 当突变为I553V时，这不与D551S组合。