公开(公告)号：US20210096128A1

公开(公告)日：2021-04-01

申请号：US17060546

申请日：2020-10-01

Applicant: Repligen Corporation

Inventor： James Ronald Peyser ,  Ramsey Shanbaky

IPC: G01N33/566 ,  G01N30/74 ,  G01N30/86 ,  G01N30/28

Abstract: The present disclosure provides systems and methods that allow users to quickly determine titer and remove hold steps by determining a first concentration using slope spectroscopy, depleting the fluid of the expressed protein by selective adsorption, and determining a second concentration using slope spectroscopy. Further, the systems and methods of the present disclosure allows the user to forgo the use of a bioanalyzer or HPLC.

公开(公告)号：US20230294021A1

公开(公告)日：2023-09-21

申请号：US18123917

申请日：2023-03-20

Applicant: Repligen Corporation

Inventor： Ramsey Shanbaky ,  Dodd Weisenberger ,  Derek Carroll ,  Paul Lalo ,  Brandon Goldberg

IPC: B01D29/90 ,  G01N15/06

CPC classification number: B01D29/908 ,  G01N15/06 ,  G01N21/33

Abstract: A system for TFF filtration of a feedstock includes a feed vessel containing a solution comprising a target biomolecule, a filter membrane for filtering the solution, a feed pump for moving the solution from the feed vessel to the filter membrane, a buffer vessel coupled to the feed vessel by a buffer supply line, and a diafiltration pump disposed in the buffer supply line for delivering a buffer solution to the feed vessel. A variable path-length instrument is coupled to the feed line for determining, in real time, a concentration of the target biomolecule in the solution. A controller is coupled to the variable path-length instrument, the feed pump, and the diafiltration pump to control operation of the system based on the received information.