专利检索 ap:("Richard Carter" OR "Martin Rosenburg" OR "Daniel R. Gentry" OR "Nigel Grinter") AND inv:"Martin Rosenburg" 第 1 页

1.

发明授权
Methods of capturing, detecting and quantifying RNA:DNA hybrids and a modified RNase H useful therein 有权
标题翻译：捕获，检测和定量RNA的方法：DNA杂交体和其中可用的修饰的RNA酶H

公开(公告)号：US07560232B2

公开(公告)日：2009-07-14

申请号：US10742355

申请日：2003-12-18

申请人： Richard Carter , Martin Rosenburg , Daniel R. Gentry , Nigel Grinter

发明人： Richard Carter , Martin Rosenburg , Daniel R. Gentry , Nigel Grinter

IPC分类号： C12Q1/68 , C07K14/00

CPC分类号： C12Q1/6816 , C12Q1/6827 , C12Q2561/108 , C12Q2522/101 , C12Q2535/131

摘要： Methods for specific RNA capture, detection and quantification are presented utilizing a protein that selectively binds RNA:DNA hybrids, preferably an RNase H that is modified to reduce degradation of the nucleic acid molecules and enhance specific detection of mixed RNA:DNA nucleic acid hybrids. Labeling of the RNA and/or amplification is not required to perform these methods. Modified RNase H enzymes useful in such methods are disclosed.

摘要翻译： 使用选择性结合RNA的蛋白质提供特异性RNA捕获，检测和定量的方法：DNA杂交体，优选核糖核酸酶H，其被修饰以减少核酸分子的降解并增强混合RNA：DNA核酸杂交体的特异性检测。 RNA和/或扩增的标记不需要执行这些方法。公开了可用于这些方法的修饰的RNase H酶。