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公开(公告)号:US06599706B1
公开(公告)日:2003-07-29
申请号:US08487453
申请日:1995-06-07
申请人: Robert F. Halenbeck , Michael Kriegler , Jan Tuttleman , Carl Perez , David A. Jewell , Kirston E. Koths
发明人: Robert F. Halenbeck , Michael Kriegler , Jan Tuttleman , Carl Perez , David A. Jewell , Kirston E. Koths
IPC分类号: G01N3353
CPC分类号: C12N9/6424 , A61K38/00 , C07K5/06191 , C07K14/525 , C07K14/811 , C07K16/40 , C12Q1/37 , G01N2333/525 , Y02A50/411
摘要: Methods and materials are disclosed for the production of purified, active recombinant human neutrophil protease, PR-3, via activation of a pro-form herein referred to as proPR-3. Human PR-3 is useful for discovering inhibitors of excessive release of mature, active TNF&agr;. Also disclosed are methods for the identification of inhibitors of the conversion of the pro-form of TNF&agr; to its mature active form.
摘要翻译: 公开了通过活化本文称为proPR-3的原型的纯化的活性重组人嗜中性粒细胞蛋白酶PR-3的方法和材料。 人类PR-3可用于发现过度释放成熟的活性TNFα的抑制剂。 还公开了用于鉴定TNFα前体转化成其成熟活性形式的抑制剂的方法。