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公开(公告)号:US20080213747A1
公开(公告)日:2008-09-04
申请号:US11533193
申请日:2006-09-19
申请人: Robert M. JONES , Sriram KUMARASWAMY , Liangde LU , Frauke RININSLAND , Kevin LEY , Wensheng XIA , Duncan MCBRANCH , David G. WHITTEN
发明人: Robert M. JONES , Sriram KUMARASWAMY , Liangde LU , Frauke RININSLAND , Kevin LEY , Wensheng XIA , Duncan MCBRANCH , David G. WHITTEN
IPC分类号: C12Q1/70 , G01N33/543 , C12Q1/68 , G01N33/53 , G01N33/552
CPC分类号: B82Y20/00 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C09B69/101 , C09B69/105 , C09B69/109 , C12Q1/6834 , G01N33/533 , G01N33/542 , G01N33/54393 , G01N33/582 , Y10T428/2991 , Y10T436/143333 , C12Q2563/107
摘要: A chemical composition including a fluorescent polymer and a receptor that is specific for both a target biological agent and a chemical moiety including (a) a recognition element, (b) a tethering element, and (c) a property-altering element is disclosed. Both the fluorescent polymer and the receptor are co-located on a support. When the chemical moiety is bound to the receptor, the property-altering element is sufficiently close to the fluorescent polymer to alter the fluorescence emitted by the polymer. When an analyte sample is introduced, the target biological agent, if present, binds to the receptor, thereby displacing the chemical moiety from the receptor, resulting in an increase of detected fluorescence. Assays for detecting the presence of a target biological agent are also disclosed.
摘要翻译: 公开了包含荧光聚合物和对靶生物剂和化学部分特异性的受体的化学组合物,包括(a)识别元件,(b)束缚元件和(c)改性元件。 荧光聚合物和受体均位于载体上。 当化学部分与受体结合时,性质改变元件足够接近荧光聚合物以改变聚合物发射的荧光。 当引入分析物样品时,目标生物试剂(如果存在)与受体结合,从而将化学部分从受体移位,导致检测到的荧光增加。 还公开了用于检测靶生物剂的存在的测定。