公开(公告)号：US06396267B1

公开(公告)日：2002-05-28

申请号：US09487929

申请日：2000-01-19

申请人： Roland Riek ,  Gerhard Wider ,  Konstantin Pervushin ,  Kurt Wuethrich

发明人： Roland Riek ,  Gerhard Wider ,  Konstantin Pervushin ,  Kurt Wuethrich

IPC分类号： G01V300

CPC分类号： G01R33/4608

摘要： A method for performing polarization transfer in NMR experiments with coupled spin ½ nuclei I and S being irradiated by a sequence of rf pulses comprising a first 90° pulse exciting the spins of the nuclei I and after a delay time a further 90° pulse exciting the spins of the nuclei S is characterized in that there is no inversion pulse acting on the spins of the nuclei S during a time period T between the first 90° pulse exciting the spins of the nuclei I and either the further 90° pulse exciting the spins of the nuclei S or a second 90° pulse acting on the spins of the nuclei I, and that the length of the time period T is chosen such that d/dT[{square root over (sinh+L (RCT+L )2+L +sin(&pgr;JIST+L )2+L )} exp(−RIT)] is minimized, where RC is the transverse cross-correlation-relaxation rate of nuclei I, RI is the total transverse relaxation rate of nuclei I and JIS is the scalar coupling constant between nuclei I and S. This provides a novel polarization transfer element which can be used as a “building block” for a great variety of complex NMR experiments including macromolecules with molecular weights far beyond 100000 and yielding higher sensitivity in comparison with methods according to the state of the art such as INEPT.

摘要翻译： 在NMR实验中进行极化转移的方法，其中耦合的自旋½核I和S被包括激发核I的自旋的第一90°脉冲的rf脉​​冲序列照射，并且在延迟时间之后进一步90°脉冲激发 核S的自旋特征在于，在激发核I的自旋的第一90°脉冲和激励自旋的另外的90°脉冲之间的时间段T期间，没有反作用脉冲作用在核S的自旋上 的核S或作用在核I的自旋上的第二90°脉冲，并且选择时间段T的长度，使得最小化，其中，C i是核I的横向互相关松弛率，RI是 核I和JIS的总横向松弛率是核I和S之间的标量耦合常数。这提供了一种新型的极化转移元件，其可以用作各种复杂NMR实验的“构建块”，包括 分子量远远超过100000的uding大分子，与根据现有技术的方法如INEPT相比，产生更高的灵敏度。