公开(公告)号：US06753185B2

公开(公告)日：2004-06-22

申请号：US10262163

申请日：2002-10-01

申请人： Ronald D. Macfarlane ,  Brian D. Hosken ,  Zachlyn N. Farwig ,  Irma L. Espinosa ,  Christine L. Myers ,  Steven L. Cockrill

发明人： Ronald D. Macfarlane ,  Brian D. Hosken ,  Zachlyn N. Farwig ,  Irma L. Espinosa ,  Christine L. Myers ,  Steven L. Cockrill

IPC分类号： G01N3392

CPC分类号： G01N33/92 ,  G01N2800/044

摘要： Methods are disclosed which separate and identify lipoproteins in biological samples. An ultracentrifuge density gradient is used to separate lipoprotein fractions. The fractions are visualized, resulting in a lipoprotein profile. The fractions can be further analyzed by a wide array of laboratory and clinical methods. The lipoprotein profile can be used in clinical diagnoses and other medical applications.

摘要翻译： 公开了在生物样品中分离和鉴定脂蛋白的方法。 使用超离心机密度梯度分离脂蛋白级分。 将级分可视化，产生脂蛋白谱。 可以通过各种实验室和临床方法进一步分析部分。 脂蛋白谱可用于临床诊断和其他医学应用。

公开(公告)号：US06593145B2

公开(公告)日：2003-07-15

申请号：US10092032

申请日：2002-03-06

申请人： Ronald D. Macfarlane ,  Brian D. Hosken

发明人： Ronald D. Macfarlane ,  Brian D. Hosken

IPC分类号： G01N930

CPC分类号： B01D17/0217 ,  G01N27/4476 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/111666 ,  Y10T436/25375

摘要： Methods of forming a density gradient by applying a centrifugal field to a solution of one or more metal ion chelate complexes are disclosed. The density gradients are self-forming equilibrium gradients and are useful for separating biological particles by ultracentrifugation. Also disclosed are methods of separating biological particles according to their density. Also disclosed are density gradients of lipoprotein particles and one or more metal ion chelate complexes, wherein the lipoprotein particles are partitioned along the density gradient according to their particle density.

公开(公告)号：US20140193846A1

公开(公告)日：2014-07-10

申请号：US14112670

申请日：2011-12-23

申请人： Ronald D. Macfarlane ,  Catherine J. McNeal ,  Subroto Chatterjee

发明人： Ronald D. Macfarlane ,  Catherine J. McNeal ,  Subroto Chatterjee

IPC分类号： G01N33/92

CPC分类号： G01N33/92 ,  C07K14/775 ,  G01N2800/324 ,  G01N2800/50

摘要： Novel isoforms of apolipoprotein C-I (apoC-I), namely apolipoprotein C-I1 (apoC-I1) and apolipoprotein C-I1′ (apoC-I1′), both of which have a molecular weight of approximately 90 daltons greater than native apolipoprotein C-I (SEQ ID NO:6) and native apolipoprotein C-I′ (SEQ ID NO:7), are shown to be both biomarkers for diagnosing atherosclerotic disease as well as risk factors for subjects having increased risk of developing an atherosclerotic disease.

摘要翻译： 载脂蛋白C1（apoC-I），即载脂蛋白C-I1（apoC-I1）和载脂蛋白C-I1'（apoC-I1'）的新型同工型，其分子量比天然载脂蛋白CI大约为90道尔顿 （SEQ ID NO：6）和天然载脂蛋白C1'（SEQ ID NO：7）显示为用于诊断动脉粥样硬化疾病的生物标志物以及具有发展动脉粥样硬化疾病风险增加的受试者的危险因素。