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公开(公告)号：US20120045828A1
公开(公告)日：2012-02-23
申请号：US13284482
申请日：2011-10-28
申请人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
发明人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
CPC分类号： G01N33/54333 , B03C1/01 , B03C1/286 , B03C2201/18 , B03C2201/26
摘要： Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
摘要翻译： 这里描述了一种自动化的机器人装置，其以非常高的纯度分离循环肿瘤细胞（CTC）或其他生物结构。 该设备使用强大的磁棒覆盖在可拆卸的塑料袖子。 这些棒穿过血液样品，捕获例如用与磁性响应颗粒例如超顺磁珠连接的抗体标记的癌细胞。 在完成捕获方案后，磁棒经历几轮洗涤，从而除去所有污染的血细胞。 通过从袖子中取出磁棒将捕获的靶细胞释放到最终的捕获溶液中。 此外，由该装置俘获的细胞在捕获后培养时显示出不降低的活力。 以适合于遗传分析的状态捕获细胞。 还公开了用于单细胞分析的方法。 机器人允许设备以高吞吐量运行。
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公开(公告)号：US09267943B2
公开(公告)日：2016-02-23
申请号：US13284482
申请日：2011-10-28
申请人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
发明人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
IPC分类号： G01N33/543 , B03C1/01 , B03C1/28
CPC分类号： G01N33/54333 , B03C1/01 , B03C1/286 , B03C2201/18 , B03C2201/26
摘要： Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
摘要翻译： 这里描述了一种自动化的机器人装置，其以非常高的纯度分离循环肿瘤细胞（CTC）或其他生物结构。 该设备使用强大的磁棒覆盖在可拆卸的塑料袖子。 这些棒穿过血液样品，捕获例如用与磁性响应颗粒例如超顺磁珠连接的抗体标记的癌细胞。 在完成捕获方案后，磁棒经历几轮洗涤，从而除去所有污染的血细胞。 通过从袖子中取出磁棒将捕获的靶细胞释放到最终的捕获溶液中。 此外，由该装置俘获的细胞在捕获后培养时显示出不降低的活力。 以适合于遗传分析的状态捕获细胞。 还公开了用于单细胞分析的方法。 机器人允许设备以高吞吐量运行。
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公开(公告)号：US08071395B2
公开(公告)日：2011-12-06
申请号：US12333213
申请日：2008-12-11
申请人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
发明人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
IPC分类号： G01N33/551
CPC分类号： G01N33/54333 , B03C1/01 , B03C1/286 , B03C2201/18 , B03C2201/26
摘要： Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
摘要翻译： 这里描述了一种自动化的机器人装置，其以非常高的纯度分离循环肿瘤细胞（CTC）或其他生物结构。 该设备使用强大的磁棒覆盖在可拆卸的塑料袖子。 这些棒穿过血液样品，捕获例如用与磁性响应颗粒例如超顺磁珠连接的抗体标记的癌细胞。 在完成捕获方案后，磁棒经历几轮洗涤，从而除去所有污染的血细胞。 通过从袖子中取出磁棒将捕获的靶细胞释放到最终的捕获溶液中。 此外，由该装置俘获的细胞在捕获后培养时显示出不降低的活力。 以适合于遗传分析的状态捕获细胞。 还公开了用于单细胞分析的方法。 机器人允许设备以高吞吐量运行。
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公开(公告)号：US20090220979A1
公开(公告)日：2009-09-03
申请号：US12333213
申请日：2008-12-11
申请人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
发明人： Ronald W. Davis , Stefanie S. Jeffrey , Michael N. Mindrinos , R. Fabian Pease , Ashley Ann Powell , AmirAli Hajhossein Talasaz
CPC分类号： G01N33/54333 , B03C1/01 , B03C1/286 , B03C2201/18 , B03C2201/26
摘要： Described here is an automated robotic device that isolates circulating tumor cells (CTCs) or other biological structures with extremely high purity. The device uses powerful magnetic rods covered in removable plastic sleeves. These rods sweep through blood samples, capturing, e.g., cancer cells labeled with antibodies linked to magnetically responsive particles such as superparamagnetic beads. Upon completion of the capturing protocol, the magnetic rods undergo several rounds of washing, thereby removing all contaminating blood cells. The captured target cells are released into a final capture solution by removing the magnetic rods from the sleeves. Additionally, cells captured by this device show no reduced viability when cultured after capture. Cells are captured in a state suitable for genetic analysis. Also disclosed are methods for single cell analysis. Being robotic allows the device to be operated with high throughput.
摘要翻译： 这里描述了一种自动化的机器人装置，其以非常高的纯度分离循环肿瘤细胞（CTC）或其他生物结构。 该设备使用强大的磁棒覆盖在可拆卸的塑料袖子。 这些棒穿过血液样品，捕获例如用与磁性响应颗粒例如超顺磁珠连接的抗体标记的癌细胞。 在完成捕获方案后，磁棒经历几轮洗涤，从而除去所有污染的血细胞。 通过从袖子中取出磁棒将捕获的靶细胞释放到最终的捕获溶液中。 此外，由该装置俘获的细胞在捕获后培养时显示出不降低的活力。 以适合于遗传分析的状态捕获细胞。 还公开了用于单细胞分析的方法。 机器人允许设备以高吞吐量运行。
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