公开(公告)号：US20140314831A1

公开(公告)日：2014-10-23

申请号：US13877101

申请日：2011-09-29

申请人： Roy Duncan ,  John Lewis ,  Roberto Justo De Antueno ,  Rae-Lynn Nesbitt ,  Eileen Kathryn Clancy

发明人： Roy Duncan ,  John Lewis ,  Roberto Justo De Antueno ,  Rae-Lynn Nesbitt ,  Eileen Kathryn Clancy

IPC分类号： C07K14/46 ,  C12N15/113

CPC分类号： C07K14/46 ,  A61K9/1271 ,  C07K2319/00 ,  C07K2319/03 ,  C07K2319/033 ,  C07K2319/33 ,  C12N15/111 ,  C12N15/113 ,  C12N2310/14 ,  C12N2320/32

摘要： Disclosed is a recombinant polypeptide for facilitating membrane fusion. The recombinant polypeptide having a sequence with at least 80% sequence identity with the ectodomain of p14 fusion-associated small transmembrane (FAST) protein and having a functional myristoylation motif, a transmembrane domain from a FAST protein and a sequence with at least 80% sequence identity with the endodomain of p15 FAST protein. A targeting ligand can be added to the recombinant polypeptide for selective fusion. The recombinant polypeptide can be included in the membrane of a liposome, or the like, to facilitate the delivery of bioactive compounds, such as siRNA, or the recombinant polypeptide can be mixed with a lipid carrier and added to cultured cells to induce cell-cell fusion and heterokaryon formation.

摘要翻译： 公开了一种促进膜融合的重组多肽。 该重组多肽具有与p14融合相关小跨膜（FAST）蛋白的胞外域具有至少80％的序列同一性并具有功能性的肉豆蔻酰化基序，来自FAST蛋白的跨膜结构域和至少80％序列的序列的序列 与p15 FAST蛋白的endodomain一致。 可以将靶向配体加入重组多肽进行选择性融合。 重组多肽可以包含在脂质体等的膜中，以促进生物活性化合物（例如siRNA）的递送，或者重组多肽可以与脂质载体混合并加入到培养的细胞中以诱导细胞细胞 融合和异核形成。

公开(公告)号：US10227386B2

公开(公告)日：2019-03-12

申请号：US13877101

申请日：2011-09-29

申请人： Roy Duncan ,  Eileen Kathryn Clancy ,  John Lewis ,  Roberto Justo De Antueno ,  Rae-Lynn Nesbitt

发明人： Roy Duncan ,  Eileen Kathryn Clancy ,  John Lewis ,  Roberto Justo De Antueno ,  Rae-Lynn Nesbitt

IPC分类号： C07K14/46 ,  C07K14/01 ,  A61K9/127 ,  C12N15/113 ,  C12N15/11

摘要： Disclosed is a recombinant polypeptide for facilitating membrane fusion. The recombinant polypeptide having a sequence with at least 80% sequence identity with the ectodomain of p14 fusion-associated small transmembrane (FAST) protein and having a functional myristoylation motif, a transmembrane domain from a FAST protein and a sequence with at least 80% sequence identity with the endodomain of p15 FAST protein. A targeting ligand can be added to the recombinant polypeptide for selective fusion. The recombinant polypeptide can be included in the membrane of a liposome, or the like, to facilitate the delivery of bioactive compounds, such as siRNA, or the recombinant polypeptide can be mixed with a lipid carrier and added to cultured cells to induce cell-cell fusion and heterokaryon formation.