公开(公告)号：US06342350B1

公开(公告)日：2002-01-29

申请号：US09148503

申请日：1998-09-04

申请人： Rudolph E. Tanzi ,  Bradley T. Hyman ,  George W. Rebeck ,  Deborah L. Blacker

发明人： Rudolph E. Tanzi ,  Bradley T. Hyman ,  George W. Rebeck ,  Deborah L. Blacker

IPC分类号： C12Q168

CPC分类号： C07K14/8107 ,  C12Q1/6883 ,  C12Q2600/156 ,  C12Q2600/172 ,  G01N33/6896 ,  G01N2800/2821

摘要： The disclosed invention relates to a diagnostic method for Alzheimer's disease based on genotyping the Alpha-2-Macroglobulin locus. A statistically significant correlation was found between inheritance of particular alleles of the Alpha-2-Macroglobulin gene and the occurrence of Alzheimer's disease. The diagnostic method involves the isolation of nucleic acid from an individual and subsequent genotyping by means such as sequencing or restriction fragment length polymorphism analysis. The invention also provides a means of genotype analysis through protein isotyping Alpha-2-Macroglobulin variant proteins. Finally, kits for nucleic acid analysis or protein analysis are taught.

摘要翻译： 所公开的发明涉及基于α-2-巨球蛋白基因座的基因分型的阿尔茨海默病的诊断方法。 发现α-2-巨球蛋白基因特异性等位基因遗传与阿尔茨海默病发生之间有统计学意义的相关性。 诊断方法包括通过诸如测序或限制性片段长度多态性分析的方法从个体和随后的基因分型中分离核酸。 本发明还提供通过蛋白质同种型分析α-2-巨球蛋白变体蛋白的基因型分析方法。 最后，教导了用于核酸分析或蛋白质分析的试剂盒。